Serological techniques

  • 文章类型: Journal Article
    植物病害对全球经济影响显著,和植物病原微生物,如线虫,病毒,细菌,真菌,和类病毒可能是大多数传染病的病因。在农业方面,无病植物的发展是决定田间植物生存和生产力的重要策略。本文综述了在其他领域得到有效应用的生物传感器检测疾病的方法,这些方法可能会改变农业的生产方式。植物病原体的精确识别有助于评估有效的管理步骤,以最大程度地减少生产损失。新的植物病原体检测方法包括疾病体征的评估,培养生物的检测,或通过分子和血清学技术直接检查受污染的组织。基于实验室的方法既昂贵又耗时,需要专业技能。这次审查的结论也表明,迫切需要建立一个可靠的,快,准确,响应,和经济有效的测试方法,用于检测生长早期的田间植物。我们还总结了新兴的生物传感器技术,包括等温扩增,纳米材料的检测,纸质技术,机器人,和芯片实验室分析设备。然而,这些构成了可持续农业中病原体早期诊断方法研究和开发的新颖性。
    Plant diseases significantly impact the global economy, and plant pathogenic microorganisms such as nematodes, viruses, bacteria, fungi, and viroids may be the etiology for most infectious diseases. In agriculture, the development of disease-free plants is an important strategy for the determination of the survival and productivity of plants in the field. This article reviews biosensor methods of disease detection that have been used effectively in other fields, and these methods could possibly transform the production methods of the agricultural industry. The precise identification of plant pathogens assists in the assessment of effective management steps for minimization of production loss. The new plant pathogen detection methods include evaluation of signs of disease, detection of cultured organisms, or direct examination of contaminated tissues through molecular and serological techniques. Laboratory-based approaches are costly and time-consuming and require specialized skills. The conclusions of this review also indicate that there is an urgent need for the establishment of a reliable, fast, accurate, responsive, and cost-effective testing method for the detection of field plants at early stages of growth. We also summarized new emerging biosensor technologies, including isothermal amplification, detection of nanomaterials, paper-based techniques, robotics, and lab-on-a-chip analytical devices. However, these constitute novelty in the research and development of approaches for the early diagnosis of pathogens in sustainable agriculture.
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