开发可以快速到达深度出血部位的水凝胶,坚持伤口,在民用和战场环境中扩张以阻止致命和/或不可压缩的出血仍然是一个挑战。在这里,注射剂,抗菌,自我扩张,报道了具有促凝活性和快速凝胶化的自推进水凝胶生物粘合剂。这种水凝胶结合了自发的气体发泡和快速的席夫碱交联,用于致命的大出血。水凝胶具有快速的凝胶化和膨胀率,高的自膨胀比,优异的抗菌活性,抗氧化效率,和组织粘附能力。此外,水凝胶具有良好的细胞相容性,促凝血能力,和更高的血细胞/血小板粘附活性比商业战斗纱布和明胶海绵。优化后的水凝胶(OD-C/QGQL-A30)在大鼠肝脏和股动脉出血模型中显示出比战斗纱布和明胶海绵更好的止血能力,有/没有抗凝剂的兔体积肝丢失大量出血模型,和兔肝肾切口出血模型,出血部位不可见。尤其是,OD-C/QGQL-A30迅速阻止兔骨盆区出血,猪锁骨下动脉静脉横断.此外,OD-C/QGQL-A30具有生物降解性和生物相容性,并加速耐甲氧西林金黄色葡萄球菌(MRSA)感染的皮肤伤口愈合。这种注射剂,抗菌,自我扩张,自推进水凝胶开辟了一条新的途径来开发致命的大量出血的止血剂,腹部器官出血,和凝血损伤出血。
Developing hydrogels that can quickly reach deep bleeding sites, adhere to wounds, and expand to stop lethal and/or noncompressible bleeding in civil and battlefield environments remains a challenge. Herein, an injectable, antibacterial, self-expanding, and self-propelling hydrogel bioadhesive with procoagulant activity and rapid gelation is reported. This hydrogel combines spontaneous gas foaming and rapid Schiff base crosslinking for lethal massive hemorrhage. Hydrogels have rapid gelation and expansion rate, high self-expanding ratio, excellent antibacterial activity, antioxidant efficiency, and tissue adhesion capacity. In addition, hydrogels have good cytocompatibility, procoagulant ability, and higher blood cell/platelet adhesion activity than commercial combat gauze and gelatin sponge. The optimized hydrogel (OD-C/QGQL-A30) exhibits better hemostatic ability than combat gauze and gelatin sponge in rat liver and femoral artery bleeding models, rabbit volumetric liver loss massive bleeding models with/without anticoagulant, and rabbit liver and kidney incision bleeding models with bleeding site not visible. Especially, OD-C/QGQL-A30 rapidly stops the bleedings from pelvic area of rabbit, and swine subclavian artery vein transection. Furthermore, OD-C/QGQL-A30 has biodegradability and biocompatibility, and accelerates Methicillin-resistant S. aureus (MRSA)-infected skin wound healing. This injectable, antibacterial, self-expanding, and self-propelling hydrogel opens up a new avenue to develop hemostats for lethal massive bleeding, abdominal organ bleeding, and bleeding from coagulation lesions.