据报道,中华二倍体是一种有趣的寄生藤壶,会对生长产生负面影响,蜕皮,在几种商业上重要的梭子蟹中繁殖。为了更好地理解宿主-寄生虫相互作用的分子机制,我们通过高通量测序方法表征了健康和D.sinensis感染的ranguinolentus的基因表达谱。完全正确,转录组学分析从感染组和对照组中产生了52、266、600和51、629、604个高质量读数,分别。干净的读数被组装到90,740和69,314个unigenes,平均长度为760bp和709bp,分别。表达分析表明,18959个基因被中华毛虫寄生显著改变,包括4769个激活基因和14,190个抑制基因。差异表达的基因分为258个KEGG途径和647个GO术语。GO分析绘制了与免疫系统过程相关的13个DEGs和与免疫反应相关的32个DEGs,分别,提示血红假单胞菌补体级联中转录表达模式的潜在改变。此外,4个代表性的蜕皮相关基因在寄生组中下调,表明D.sinensis感染似乎抑制了蜕皮类固醇激素的产生。总之,本研究提高了我们对寄生虫-宿主相互作用机制的理解,专注于蜕皮激素受体的功能,甲壳蟹类寄生虫感染的Toll样受体和细胞因子受体。
Diplothylacus sinensis is reported as an intriguing parasitic barnacle that can negatively affect the growth, molting, reproduction in several commercially important portunid crabs. To better understand the molecular mechanisms of host-parasite interactions, we characterized the gene expression profiles from the healthy and D. sinensis infected Portunus sanguinolentus by high-through sequence method. Totally, the transcriptomic analysis generated 52, 266, 600 and 51, 629, 604 high quality reads from the infected and control groups, respectively. The clean reads were assembled to 90,740 and 69,314 unigenes, with the average length of 760 bp and 709 bp, respectively. The expression analysis showed that 18,959 genes were significantly changed by the parasitism of D. sinensis, including 4769 activated genes and 14,190 suppressed genes. The differentially expressed genes were categorized into 258 KEGG pathways and 647 GO terms. The GO analysis mapped 13 DEGs related to immune system process and 32 DEGs related to immune response, respectively, suggesting a potential alteration of transcriptional expression patterns in complement cascades of P. sanguinolentus. Additionally, 4 representative molting-related genes were down-regulated in parasitized group, indicating D. sinensis infection appeared to suppress the producing of ecdysteroid hormones. In conclusion, the present study improves our understanding on parasite-host interaction mechanisms, which focuses the function of Ecdysone receptor, Toll-like receptor and cytokine receptor of crustacean crabs infestation with rhizocephalan parasites.