评价脱细胞胶原支架(ACS)联合碱性成纤维细胞生长因子(bFGF)修复大鼠外伤性鼓膜(TM)穿孔的效果。
创伤性TM穿孔大鼠模型的前瞻性对照动物研究。
三级医疗中心。
Sprague-Dawley大鼠(N=84)的右侧TMs单侧外伤性穿孔随机接受ACS,bFGF,ACS联合bFGF(ACS/bFGF),或者什么都没有(自发愈合,没有任何干预作为对照组)。通过耳镜检查评估愈合结果,光学相干层析成像,组织学,和透射电子显微镜在术后1、2和4周。通过听觉脑干反应测试评估听力结果。
ACS/bFGF在早期比自发愈合更高的穿孔闭合率,ACS,和bFGF。根据组织学,光学相干层析成像,和透射电子显微镜,具有成熟的三层结构和均匀的厚度,ACS/bFGF组胶原纤维密集堆积。听觉脑干反应评估还显示,与对照组相比,ACS/bFGF治疗可促进更快的功能性听力恢复。
ACS是有效的TM支架和bFGF的载体。ACS/bFGF提高了TM闭合率,导致重建更好的TM,并提高听力。ACS/bFGF作为临床环境中TM穿孔的潜在替代品。
To evaluate the efficacy of acellular collagen scaffold (ACS) in combination with basic fibroblast growth factor (bFGF) for the repair of traumatic tympanic membrane (TM) perforation in a rat model.
A prospective controlled animal study in a rat model of traumatic TM perforation.
Tertiary medical center.
Sprague-Dawley rats (N = 84) with unilateral traumatic perforation of the right TMs were randomized to receive ACS, bFGF, ACS in combination with bFGF (ACS/bFGF), or nothing (spontaneous healing without any interventions as a control group). The healing outcomes were evaluated by
otoscopy, optical coherence tomography, histology, and transmission electron microscopy at 1, 2, and 4 weeks postoperatively. The hearing outcomes were assessed with auditory brainstem response testing.
ACS/bFGF resulted in higher perforation closure rates at an earlier stage than spontaneous healing, ACS, and bFGF. Based on histology, optical coherence tomography, and transmission electron microscopy, a trilaminar structure and uniform thickness with mature, densely packed collagen fibers were seen in the ACS/bFGF group. Auditory brainstem response evaluation also showed that ACS/bFGF treatment promoted faster functional hearing recovery as compared with the control group.
ACS is an effective TM scaffold and a carrier for bFGF. ACS/bFGF improves the TM closure rate, results in better-reconstructed TMs, and improves hearing. ACS/bFGF serves as a potential substitute for TM perforations in clinical settings.