目的:我们研究了系统性红斑狼疮(SLE)患者的血浆沉默酶1(SIRT1)水平,并讨论了血浆SIRT1作为SLE生物标志物的潜力。
方法:共有359名受试者,包括299名患者(89名SLE,50类风湿性关节炎,30骨关节炎,30痛风,38干燥综合征,20强直性脊柱炎,30混合性结缔组织病,招募了12名系统性硬化症)和60名健康对照。酶联免疫吸附法检测SLE患者血浆中SIRT1。SIRT1水平之间的关系,临床,讨论了SLE患者的实验室特征。通过受试者工作特征(ROC)曲线分析评估血浆SIRT1可区分不同风湿性疾病患者和健康对照的SLE。
结果:与健康对照组相比,SLE患者SIRT1水平升高(6.28[5.89-6.68]vs2.42[2.10-2.74]ng/mL,P<.001)。血浆中SIRT1浓度与疾病活动显著相关(rs=.317,P<.001)。ROC曲线下面积(AUC)分析显示,与健康对照组相比,SIRT1对SLE有较好的诊断能力(AUC=0.986,P<.001)。与类风湿性关节炎相比,骨关节炎,干燥综合征,强直性脊柱炎,混合性结缔组织病和系统性硬化症患者,SLE患者血浆SIRT1的AUC分别为0.982、0.881、0.810、0.860、0.781、0.889、0.736。在评价SIRT1鉴别能力时,SLE与非SLE患者鉴别的敏感性和特异性为95.51%,61.43%,分别,在4.323ng/mL的最佳临界值。
结论:SLE患者循环SIRT1升高,可能是一个有前途的SLE诊断标记。
OBJECTIVE: We investigated plasma sirtuin-1 (SIRT1) levels in systemic lupus erythematosus (SLE) patients, and discussed potential of plasma SIRT1 as a biomarker for SLE.
METHODS: A total of 359 subjects, including 299 patients (89 SLE, 50 rheumatoid arthritis, 30 osteoarthritis, 30 gout, 38 Sjögren\'s syndrome, 20 ankylosing spondylitis, 30 mixed connective tissue disease, 12 systemic sclerosis) and 60 healthy controls were recruited. SIRT1 in plasma of SLE patients was detected by enzyme-linked immunosorbent assay. Relationship between SIRT1 levels, clinical, laboratory characteristics in SLE patients was discussed. Plasma SIRT1 to discriminate SLE from different rheumatic patients and healthy controls was assessed by receiver operating characteristic (ROC) curve analysis.
RESULTS: SIRT1 levels were elevated in SLE patients compared with healthy controls (6.28 [5.89-6.68] vs 2.42 [2.10-2.74] ng/mL, P < .001). SIRT1 concentration in plasma was significantly associated with disease activity (rs = .317, P < .001). Area under the ROC curve (AUC) analysis showed that compared to healthy controls, SIRT1 had a good ability for diagnosis of SLE (AUC = 0.986, P < .001). Compared with rheumatoid arthritis, osteoarthritis, Sjögren\'s syndrome, ankylosing spondylitis, mixed connective tissue disease and systemic sclerosis patients, the AUC of plasma SIRT1 in SLE patients was 0.982, 0.881, 0.810, 0.860, 0.781, 0.889, 0.736, respectively. When evaluating the discriminative power of SIRT1, the sensitivity and specificity for distinguishing SLE from non-SLE patients were 95.51%, 61.43%, respectively, at the optimal cut-off value of 4.323 ng/mL.
CONCLUSIONS: Circulating SIRT1 was elevated in SLE, and might be a promising SLE diagnostic marker.