化疗是治疗转移性肺癌的主要方法之一。虽然受限于抗癌药物的低肿瘤递送功效。由于其高免疫刺激作用,细菌疗法正在出现用于癌症治疗;然而,过度产生的免疫原性会引起严重的炎症反应综合征。这里,我们通过逐层封装制备了肿瘤细胞膜包被的脂质体载紫杉醇细菌鬼影(LP@BG@CCM),用于治疗转移性肺癌.制备工艺简单,只涉及成膜,电穿孔,和孔隙挤压。LP@BG@CCM比LP@BG具有更高的4T1癌细胞毒性,因为它与癌细胞的融合更快。在4T1乳腺癌转移性肺癌小鼠模型中,观察到静脉内注射LP@BG@CCM的明显更高的肺靶向性,肺外观几乎恢复正常,肺重量减少,清晰的肺组织结构,与其前体相比,癌细胞凋亡增强。此外,LP@BG@CCM后几个主要免疫因子得到改善,包括脾脏中的CD4+/CD8a+T细胞和TNF-α,IFN-γ,和IL-4在肺中。LP@BG@CCM表现出最佳的协同化学免疫疗法,这是一种治疗转移性肺癌的有前途的药物。
Chemotherapy is one of the major approaches for the treatment of metastatic lung cancer, although it is limited by the low tumor delivery efficacy of anticancer drugs. Bacterial therapy is emerging for cancer treatment due to its high immune stimulation effect; however, excessively generated immunogenicity will cause serious inflammatory response syndrome. Here, we prepared cancer cell membrane-coated liposomal paclitaxel-loaded bacterial ghosts (LP@BG@CCM) by layer-by-layer encapsulation for the treatment of metastatic lung cancer. The preparation processes were simple, only involving film formation, electroporation, and pore extrusion. LP@BG@CCM owned much higher 4T1 cancer cell toxicity than LP@BG due to its faster fusion with cancer cells. In the 4T1 breast cancer metastatic lung cancer mouse models, the remarkably higher lung targeting of intravenously injected LP@BG@CCM was observed with the almost normalized lung appearance, the reduced lung weight, the clear lung tissue structure, and the enhanced cancer cell apoptosis compared to its precursors. Moreover, several major immune factors were improved after administration of LP@BG@CCM, including the CD4+/CD8a+ T cells in the spleen and the TNF-α, IFN-γ, and IL-4 in the lung. LP@BG@CCM exhibits the optimal synergistic chemo-immunotherapy, which is a promising medication for the treatment of metastatic lung cancer.