曼陀罗(茄科)传统上在西非用于治疗哮喘,癫痫,类风湿性关节炎,丝虫病微生物感染和结膜炎。本研究研究了D.stramoniumL.(ASEDS)种子水提取物对Wistar大鼠的免疫调节作用。
将30只Wistar白化病大鼠(180-200g)随机分为6组(n=5)。第1组仅接受蒸馏水。第2-6组的大鼠用10mg/kg体重(b.w.)环磷酰胺口服预处理27天以诱导免疫抑制。此后,他们接受口服治疗28天,如下:第2组(蒸馏水),第3组(5mg/kgb.w.左旋咪唑),第4-6组(60、90和120mg/kgb.w.ASEDS,分别)。HPLC用于测定ASEDS中的主要化合物。ASEDS对免疫细胞的影响,免疫球蛋白A,G和M等级,脂蛋白,并对大鼠的抗氧化状态进行评价。
ASEDS表明阿克胺含量高,奎宁,儿茶素,绿原酸,没食子酸,槲皮素,香草酸,木犀草素,FormosaninC,皂苷,Cyanidin,单宁酸,3-Carene,柠檬烯和α-松油醇。环磷酰胺引起白细胞总数和差异显著(p<0.05)减少,IgA,IgG,高密度脂蛋白(HDL),过氧化氢酶,超氧化物歧化酶,谷胱甘肽过氧化物酶,维生素A,未处理大鼠的C和E水平。ASEDS的施用导致免疫细胞计数的显著(p<0.05)改善,免疫球蛋白合成,高密度脂蛋白浓度,治疗组大鼠的抗氧化状态。
从研究中获得的结果表明ASEDS的免疫调节活性,从而表明其在免疫刺激药物发现中的潜力。
Datura stramonium L. (Solanaceae) is used traditionally in west Africa to treat asthma, epilepsy, rheumatoid arthritis, filariasis microbial infections and conjunctivitis. This study investigated the immunomodulatory effects of aqueous seed extract of D. stramonium L. (ASEDS) on Wistar rats.
Thirty Wistar albino rats (180-200 g) were randomized into 6 groups (n = 5). Group 1 received distilled water only. Rats in groups 2-6 were pretreated with 10 mg/kg body weight (b.w.) Cyclophosphamide orally for 27-days to induce immunosuppression. Thereafter, they received treatment orally for 28 days as follows: Group 2 (distilled water), group 3 (5 mg/kg b.w.
Levamisole), groups 4-6 (60, 90 and 120 mg/kg b.w. ASEDS, respectively). HPLC was used to determine major compounds in ASEDS. The effects of ASEDS on immune cells, immunoglobulins A, G and M levels, lipoproteins, and antioxidant status of rats were evaluated.
ASEDS indicated high content of Acutumine, Quinine, Catechin, Chlorogenic acid, Gallic acid, Quercetin, Vanillic acid, Luteolin, Formosanin C, Saponin, Cyanidin, Tannic acid, 3-Carene, Limonene and α-terpineol. Cyclophosphamide triggered significant (p < 0.05) reduction in total leucocyte count and differentials, IgA, IgG, high-density lipoproteins (HDL), catalase, superoxide dismutase, glutathione peroxidase, vitamins A, C and E levels of untreated rats. Administration of ASEDS led to significant (p < 0.05) improvement in immune cell counts, immunoglobulin synthesis, high-density lipoprotein concentration, and antioxidant status of rats in the treated groups.
The results obtained from the study showed the immunomodulatory activity of ASEDS, thereby indicating its potential in immunostimulatory drug discovery.