溶血磷脂酰胆碱（LPC）已被广泛用作动物饲料中的乳化剂，以提高脂质的利用率。然而,LPC对圆角质量的影响鲜为人知。本研究是首次研究鱼类肌肉脂质组学对膳食LPC补充的反应。在56天喂养试验后收集大菱鱼肌肉样品,其中实验饮食含有0或0.25%LPC。在脂质组学分析中使用靶向串联质谱。总共62个单独的脂质(58个被LPC上调,7个被LPC下调)显示响应于膳食LPC的浓度的显著差异。这些差异丰富的脂质大多数是二酰基甘油，游离脂肪酸和心磷脂,它们都被饮食LPC上调。然而,LPC仅对肌肉脂肪酸组成和脂质含量产生边际影响。在鱼产品评估中，饮食LPC对鱼片脂质组成的影响不可忽略。
Lysophosphatidylcholine (LPC) has been widely used as emulsifier in animal feeds to enhance the lipid utilization. However, the effects of LPC on fillet quality has rarely been known. The present study was the first time to investigate the response of fish muscle lipidomics to dietary LPC supplementation. Turbot muscle samples were collected after a 56-day feeding trial where the experimental diet contained 0 or 0.25% LPC. Targeted tandem mass spectrometry was used in the lipidomic analysis. A total of 62 individual lipids (58 up-regulated and 7 down-regulated by LPC) showed significant difference in concentration in response to dietary LPC. Most of these differentially abundant lipids were diacylglycerol, free fatty acid and cardiolipin, and they all were up-regulated by dietary LPC. However, LPC exerted only marginal effects on muscle fatty acid composition and lipid content. The effects of dietary LPC on fillet lipid composition cannot be neglected in fish product evaluation.
PDF(Pubmed)