黄病毒表现出广泛的遗传多样性并表现出不同的宿主关系。蚊媒黄病毒最近在世界范围内被分离和表征。中国云南省是物种多样性最丰富的地区之一,也是亚洲大陆多物种进化的中心,它支持许多节肢动物传播的病毒(虫媒病毒)的传播。在虫媒病毒的筛查程序中,2007年至2010年云南省蚊子活动季节采集蚊子。11株黄病毒,命名为云南库蚊黄病毒(YNCxFVs),是从三带囊状库蚊和中华按蚊标本中获得的。基于部分非结构蛋白(NS)5基因的序列分析表明,YNCxFV彼此之间具有92.8-99.6%的核苷酸同一性,并且与库蚊相关的黄病毒相似。一个代表性分离株的完整基因组,对LSFlaviV-A20-09进行测序。基因组长10,865个核苷酸,包含一个单一的,10,080个核苷酸的长开放阅读框(ORF),编码3360-aa多蛋白。该基因组与广平病毒(QBV)VN180株最密切相关,从越南库蚊中分离出的一种昆虫特异性黄病毒,但ORF序列只有83.0%的核苷酸和93.8%的氨基酸同一性。基因组与其他库蚊黄病毒具有约66.3%-68.5%的核苷酸序列和69.3-73.3%的氨基酸序列同一性,只有47.9-57.9%的核苷酸序列和38.7-55.1%的氨基酸序列同一性与Coquillettidia相关,曼索尼亚相关和伊蚊相关的黄病毒。系统发育分析表明,LSFlaviV-A20-09落入了与库蚊相关的黄病毒进化枝。我们的发现提供了有关感染蚊子的病毒异质性的更多信息。
Flaviviruses present a wide range of genetic diversity and exhibit diverse host relationships. Mosquito-borne flaviviruses have recently been isolated and characterized worldwide. Yunnan Province of
China is one of the richest areas of species diversity and is the center of multi-species evolution in mainland Asia, which supports the circulation of numerous arthropod-borne viruses (arboviruses). In a screening program of arboviruses, mosquitoes were collected during the mosquito activity season in the Yunnan Province from 2007 to 2010. Eleven flavivirus strains, named Yunnan Culex flaviviruses (YNCxFVs), were obtained from Culex tritaeniorhynchus and Anopheles sinensis specimens. Sequence analyses based on partial nonstructural protein (NS) 5 gene indicated that the YNCxFVs shared 92.8-99.6% nucleotide identity with each other and were similar to the Culex-related flaviviruses. The complete genome of one representative isolate, LSFlaviV-A20-09, was sequenced. The genome was 10,865 nucleotides long and contained a single, long open reading frame (ORF) of 10,080 nucleotides that encoded a 3360-aa polyprotein. This genome was most closely related to the Quang Binh virus (QBV) VN180 strain, an insect-specific flavivirus isolated from Culex mosquitoes in Vietnam, but only had 83.0% nucleotide and 93.8% amino acid identities for the ORF sequence. The genome has approximately 66.3%-68.5% nucleotide sequence and 69.3-73.3% amino acid sequence identities to other Culex flaviviruses, and only has 47.9-57.9% nucleotide sequence and 38.7-55.1% amino acid sequence identities to Coquillettidia-related, Mansonia-related and Aedes-related flaviviruses. Phylogenetic analyses revealed that the LSFlaviV-A20-09 fell into the Culex-related flavivirus clade. Our discoveries provide more information regarding the heterogeneity of viruses that infect mosquitoes.