目的:研究白细胞介素(IL)-1α是否以及如何参与慢性鼻-鼻窦炎伴鼻息肉(CRSwNP)。
方法:从CRSwNP患者和对照受试者收集鼻息肉(NP)和对照组织。IL-1α和其他促炎细胞因子(IL-1β,IL-8和IL-13等。),以及嗜中性粒细胞和嗜酸性粒细胞的积累,使用免疫组织化学(IHC)检查鼻窦组织,免疫荧光(IF)染色,qPCR,和Luminex,分别。此外,在培养的鼻上皮细胞(NECs)中评估了IL-1α表达的调节及其对其他促炎细胞因子的影响。
结果:NP组织中IL-1α的mRNA和蛋白水平明显高于对照组织。IL-1α在息肉组织中主要位于上皮细胞和中性粒细胞。息肉IL-1α水平与IL-8、IL-1β、IL-6,IL-4和IL-13的产生,以及组织中性粒细胞浸润。此外,聚(I:C),脂多糖,鞭毛蛋白,R848和细胞因子(IL-4,IL-5和IL-13)显着增加了体外培养的NECs中IL-1α的表达,和重组IL-1α显著促进培养的NECs中IL-8和CXCL1的产生。
结论:这些发现提供了证据,表明NP组织中IL-1α显著增加,这可能导致中国CRSwNP患者的组织中性粒细胞增多。
OBJECTIVE: To examine whether and how interleukin (IL)-1α is involved in chronic rhinosinusitis with nasal polyps (CRSwNP).
METHODS: Nasal polyp (NP) and control tissues were collected from CRSwNP patients and control subjects. The expression of IL-1α and other proinflammatory cytokines (IL-1β, IL-8 and IL-13, etc.), as well as neutrophil and eosinophil accumulation, were examined in sinonasal tissues using immunohistochemical (IHC), immunofluorescent (IF) staining, qPCR, and Luminex, respectively. Moreover, the regulation of IL-1α expression and its effects on other proinflammatory cytokines were evaluated in cultured nasal epithelial cells (NECs).
RESULTS: The mRNA and protein levels of IL-1α were significantly higher in NP tissues compared to that in control tissues. IL-1α in polyp tissues was mainly located in epithelial cells and neutrophils. Polyps IL-1α level was significantly associated with IL-8, IL-1β, IL-6, IL-4 and IL-13 production, as well as tissue neutrophil infiltration. Moreover, poly (I:C), lipopolysaccharides, Flagellin, R848 and cytokines (IL-4, IL-5, and IL-13) significantly increased the expression of IL-1α in cultured NECs in vitro, and recombinant IL-1α significantly promoted production of IL-8 and CXCL1 in cultured NECs.
CONCLUSIONS: These findings provided the evidence that IL-1α were significantly increased in NP tissues, which may contribute to tissue neutrophilia in CRSwNP patients in
China.