沙门氏菌可以抵抗各种压力并在食品加工过程中存活,storage,和分配,给消费者带来潜在的健康风险。因此,评估细菌在各种环境胁迫下的生存状况是必要的。在这项研究中,五种沙门氏菌分离株的抗性谱[Agona血清型,婴儿,鼠伤寒,肠炎,和标准菌株(ATCC13076,肠炎血清型)]至酸性,高渗,并检查了氧化应激,还评估了非编码小RNA的相对表达,包括CyaR,MicC,MicA,InvR,RybB,还有DSRA,由特定应力引起。结果表明,尽管所有测试的菌株都表现出一定的抗应力能力,菌株之间的抗逆性差异很大。根据暴露于压力3小时的细胞数量的减少,肠炎杆菌对酸性和高渗胁迫的抵抗力最高,而ATCC13076对氧化应激表现出最大的抵抗力,细胞减少量小于0.1LogCFU/ml。细胞对酸性有更大的抵抗力,高渗,在1小时内观察到氧化应激,2小时后,从1到2小时,分别。sRNAs的相对表达取决于每种胁迫的分离株;受试分离株的酸性暴露诱导了DsrA的高表达水平,MicC,InvR,RybB,MicA,还有CyaR.sRNARybB,与细菌中的sigmaE和外膜蛋白相关,显示暴露于测试应力的肠炎沙门氏菌的倍数变化大于7。参与一般应激反应和应激适应的CyaR和InvR也被诱导显示暴露于高渗应激的沙门氏菌的高表达水平。总的来说,这些发现表明,沙门氏菌在特定应力下的行为随应变而变化,并且可能与其他特征无关。该发现还为沙门氏菌在短期压力下的生存以及在食品工业中控制沙门氏菌提供了见解。
Salmonella can resist various stresses and survive during food processing, storage, and distribution, resulting in potential health risks to consumers. Therefore, evaluation of bacterial survival profiles under various environmental stresses is necessary. In this study, the resistance profiles of five Salmonella isolates [serotypes with Agona, Infantis, Typhimurium, Enteritidis, and a standard strain (ATCC 13076, Enteritidis serotype)] to acidic, hyperosmotic, and oxidative stresses were examined, and the relative expressions of non-coding small RNAs were also evaluated, including CyaR, MicC, MicA, InvR, RybB, and DsrA, induced by specific stresses. The results indicated that although all tested strains displayed a certain resistance to stresses, there was great diversity in stress resistance among the strains. According to the reduction numbers of cells exposed to stress for 3 h, S. Enteritidis showed the highest resistance to acidic and hyperosmotic stresses, whereas ATCC 13076 showed the greatest resistance to oxidative stress, with less than 0.1 Log CFU/ml of cell reduction. Greater resistance of cells to acidic, hyperosmotic, and oxidative stresses was observed within 1 h, after 2 h, and from 1 to 2 h, respectively. The relative expression of sRNAs depended on the isolate for each stress; acidic exposure for the tested isolates induced high expression levels of DsrA, MicC, InvR, RybB, MicA, and CyaR. The sRNA RybB, associated with sigma E and outer membrane protein in bacteria, showed a fold change of greater than 7 in S. Enteritidis exposed to the tested stresses. CyaR and InvR involved in general stress responses and stress adaptation were also induced to show high expression levels of Salmonella exposed to hyperosmotic stress. Overall, these findings demonstrated that the behaviors of Salmonella under specific stresses varied according to strain and were likely not related to other profiles. The finding also provided insights into the survival of Salmonella subjected to short-term stresses and for controlling Salmonella in the food industry.