一种多功能和生物相容性混合微凝胶（聚（VPBA-AAm）-CD），使用N，S掺杂碳点（CD）和乙二醇二甲基丙烯酸酯（EGDMA）作为交联剂，以4-乙烯基苯硼酸（VPBA）和丙烯酰胺（AAm）为单体，是在这项工作中设计的。使用合理设计的氢键键合络合物方法，通过反应物的简单一锅自由基分散聚合可以很容易地制备这种微凝胶。杂化微凝胶是具有光滑表面和234±8nm的平均粒度的球形颗粒。聚（VPBA-AAm）-CD微凝胶在生理pH值下在临床关注的范围内表现出葡萄糖响应性溶胀，并可以实现胰岛素的可控释放。此外,混合微凝胶（聚（VPBA-AAm）-CD）中胰岛素的释放速率可以由溶液中的葡萄糖浓度触发，葡萄糖浓度的增加可以加速胰岛素的释放。进一步的体外细胞毒性研究表明，微凝胶具有良好的生物相容性，对细胞无明显毒性。这些表明制备的微凝胶（聚（VPBA-AAm）-CD）可能为糖尿病胰岛素缺乏症的自我调节治疗提供新的模式。
A multifunctional and biocompatible hybrid microgel (poly(VPBA-AAm)-CD) using N, S-doped carbon dots (CDs) and ethylene glycol dimethacrylate (EGDMA) as cross-linking agents, and 4-vinylbenzene boronic acid (VPBA) and acrylamide (AAm) as monomers, was designed in this work. This microgel can be easily prepared by a simple one-pot radical dispersion polymerization of the reactants using a rationally designed hydrogen-bonded complex method. The hybrid microgels were spherical particles with a smooth surface and an average particle size of 234 ± 8 nm. The poly(VPBA-AAm)-CD microgel displayed the glucose-responsive swelling within a clinically concerned range at a physiological pH and could realize the controllable release of insulin. In addition, the release rate of insulin in the hybrid microgel (poly(VPBA-AAm)-CD) could be triggered by glucose concentrations in the solution, and the increasing glucose concentrations can accelerate the insulin release. Further in vitro cytotoxicity studies showed that the microgel had good biocompatibility and no obvious toxicity to the cells. These indicate that the prepared microgel (poly(VPBA-AAm)-CD) may supply a new pattern for the self-regulating therapy of insulin deficiency in diabetes.
