1,3-丙二醇是生产聚对苯二甲酸丙二醇酯(PTT)的重要单体。目前,它主要由微生物发酵生产,which,然而,生产效率低。为了解决这个问题,本研究采用大气室温等离子体(ARTP)诱变技术和高通量筛选,获得一株对渗透压具有高耐受性的菌株,1,3-丙二醇的效价为87g/L。此外,筛选出适合肺炎克雷伯菌的基因表达元件,和代谢工程被用来阻断冗余的代谢途径(ldhA的缺失,buda,和aldA)并增强合成途径(dhaB和yqhD的过表达)。该工程菌株产生的1,3-丙二醇的滴度增加到107g/L。最后,在一个5升的发酵罐里,最佳菌株KP-FMME-6的1,3-丙二醇滴度为118g/L,甘油转化率为42%,生产率为2.46g/(h·L),优化后的发酵参数。本研究为工业化生产1,3-丙二醇提供了参考。
1, 3-propanediol is an important monomer for the production of polytrimethylene terephthalate (PTT). Currently, it is mainly produced by microbial fermentation, which, however, has low production efficiency. To address this problem, this study employed atmospheric room temperature plasma (ARTP) mutagenesis technology and high-throughput screening to obtain a strain with high tolerance to osmotic pressure, which achieved a 1, 3-propanediol titer of 87 g/L. Furthermore, the gene expression elements suitable for Klebsiella pneumoniae were screened, and metabolic engineering was employed to block redundant metabolic pathways (deletion of ldhA, budA, and aldA) and enhance the synthesis pathway (overexpression of dhaB and yqhD). The titer of 1, 3-propanediol produced by the engineered strain increased to 107 g/L. Finally, in a 5 L fermenter, the optimal strain KP-FMME-6 achieved a 1, 3-propanediol titer of 118 g/L, with a glycerol conversion rate of 42% and productivity of 2.46 g/(h·L), after optimization of the fermentation parameters. This study provides a reference for the industrial production of 1, 3-propanediol.