乙型疱疹病毒1型(EHV-1)在中国流行,并对马业造成显著的经济损失。然而,没有关于中国菌株的分子特征和致病性的信息。因此,一种EHV-1菌株,命名为YM2019,是从新疆流产的马胎儿的肺组织中分离出来的,中国,并对其基因组和致病性进行了分析。该分离株的全基因组长度为150,267个碱基对,G+C含量为56.7%。遗传和系统发育分析表明,菌株YM2019(GenBank:MT063054)属于ORF30N752基因型,但与英国分离的菌株Ab4(ORF30D752,GenBank:AY665713)具有很高的相似性。当将菌株YM2019与参考菌株Ab4和V592(ORF30N752,GenBank:AY464052)进行比较时,发现了十四个独特的氨基酸突变。感染YM2019菌株的叙利亚仓鼠表现出严重的呼吸道和神经系统临床症状并死亡。伊犁马感染菌株YM2019引起鼻肺炎,病毒血症,和神经系统临床症状,如后肢跛行,虚脱,减少运动。这里,我们描述了在中国发现的EHV-1菌株的特征,连同完整的基因组序列,并揭示非神经毒株(ORF30N752)也可以引起马的神经系统症状。这些数据将为进一步研究病毒基因型与致病性之间的关系提供参考。
Equid alphaherpesvirus 1 (EHV-1) is prevalent in
China, and causes notable economic damage to the equine industry. However, there is no information regarding the molecular characteristics and pathogenicity of the Chinese strains. Therefore, an EHV-1 strain, named YM2019, was isolated from the lung tissue of an aborted horse fetus in Xinjiang,
China, and its genome and pathogenicity were analyzed. The full genome of the isolate was 150,267 base pairs in length, with 56.7% G + C content. Genetic and phylogenetic analysis showed that strain YM2019 (GenBank: MT063054) belonged to the ORF30 N752 genotype but displayed a high level of similarity with strain Ab4 (ORF30 D752, GenBank: AY665713) isolated in Britain. Fourteen unique amino acid mutations were found when comparing strain YM2019 with the reference strains Ab4 and V592 (ORF30 N752, GenBank: AY464052). Syrian hamsters infected with strain YM2019 exhibited severe respiratory and neurological clinical signs and died. Infection with strain YM2019 in Yili horses caused rhinopneumonitis, viremia, and neurological clinical signs such as hind limb lameness, prostration, and reduced movement. Here, we describe the features of an EHV-1 strain discovered in
China, together with the complete genome sequence, and reveal that a nonneurovirulent strain (ORF30 N752) can also cause neurological signs in horses. The data will be useful in providing some reference for further research into the relationship between viral genotypes and pathogenicity.