人类免疫缺陷病毒1型CRF59_01B,2013年在中国确定,已在全国范围内检测到,在广州及其附近地区表现出明显的高患病率。这项研究旨在揭示其起源和迁移。建立了一套数据集,纳入广州和公共数据库中所有可用的CRF59_01Bpol基因序列及其元数据。贝叶斯系统地理分析表明CRF59_01B起源于深圳,邻近的城市广州,大约在1998年,后验概率为0.937。分子网络分析检测到1131个传输链路,并显示出极高的聚类率(78.9%)。大量的城市间传输(26.5%,300/1131)在深圳和广州之间观察到,而区域间的传播将广州与中国南方(46)和西南(64)联系起来。广州的中心是CRF59_01B传输的枢纽,包括深圳流入(3.57事件/年)和广州郊区流出(>2事件/年)。大规模分析显示,从深圳到广州(5.08事件/年)和华北(0.59事件/年)的迁移显著,并从广州传播到中部(0.47个事件/年),东部(0.42个事件/年),南方(0.76个事件/年),西南地区(0.76个事件/年)和深圳(1.89个事件/年)。深圳和广州是CRF59_01B流通的发源地和枢纽,强调城市间合作和数据共享,以限制其在全国范围内的传播。
Human immunodeficiency virus type 1 CRF59_01B, identified in
China in 2013, has been detected nationwide, exhibiting notably high prevalence in Guangzhou and its vicinity. This study aimed to unravel its origin and migration. A data set was established, incorporating all available CRF59_01B pol gene sequences and their metadata from Guangzhou and the public database. Bayesian phylogeographic analysis demonstrated that CRF59_01B originated in Shenzhen, the neighboring city of Guangzhou, around 1998 with posterior probability of 0.937. Molecular network analysis detected 1131 transmission links and showed a remarkably high clustering rate (78.9%). Substantial inter-city transmissions (26.5%, 300/1131) were observed between Shenzhen and Guangzhou while inter-region transmissions linked Guangzhou with South (46) and Southwest (64)
China. The centre of Guangzhou was the hub of CRF59_01B transmission, including the inflow from Shenzhen (3.57 events/year) and outflow to the outskirts of Guangzhou (>2 events/year). The large-scale analysis revealed significant migration from Shenzhen to Guangzhou (5.08 events/year) and North
China (0.59 events/year), and spread from Guangzhou to Central (0.47 events/year), East (0.42 events/year), South (0.76 events/year), Southwest
China (0.76 events/year) and Shenzhen (1.89 events/year). Shenzhen and Guangzhou served as the origin and the hub of CRF59_01B circulation, emphasizing inter-city cooperation and data sharing to confine its nationwide diffusion.