目的:建立Friend鼠白血病病毒(FLV)诱导的免疫缺陷BALB/C小鼠模型,观察扶正排毒片对小鼠体重的影响。胸腺,脾,脾FLV感染小鼠的CD4+和CD8+T淋巴细胞。FLV在BALB/c小鼠中传代两次。将感染的小鼠基于其体重分成六组,每组十只小鼠。各组包括正常对照组;病毒对照组;AZT组;高(2.8g/kg),培养基(1.4g/kg),和低剂量(0.7g/kg)扶正排毒片组;扶正排毒汤(10g/kg)组。小鼠经灌胃给药扶正排毒片21天。体重和胸腺的变化,脾,脾并测定每只小鼠的CD4+和CD8+T淋巴细胞。
结果:病毒对照组的脾脏重量明显高于正常对照组,有明显的脾肿大。此外,AZT组和高、中剂量扶正排毒片组的脾抑制指数约为93.80%,37.80%,和28.07%,分别。此外,高、中剂量扶正排毒片能增加感染小鼠的胸腺重量。
结论:扶正排毒片可抑制脾肿大,降低脾指数,并增加FLV诱导的免疫缺陷小鼠的胸腺重量和胸腺指数。
OBJECTIVE: To establish a Friend murine leukemia virus (FLV)-induced immunodeficient BALB/C mouse model and investigate the effects of Fuzheng Paidu tablets on the body weight, thymus, spleen, and CD4+ and CD8+ T lymphocytes of FLV-infected mice. FLV was passaged twice in BALB/c mice. The infected mice were divided into six groups of ten mice based on their weights. The groups included the normal control group; virus control group; AZT group; high- (2.8 g/kg), medium- (1.4 g/kg), and low-dose (0.7 g/kg) Fuzheng Paidu tablet groups; and Fuzheng Paidu decoction (10 g/kg) group. The mice were administered Fuzheng Paidu tablets via gavage for 21 days. The body weight and changes in the thymus, spleen, and CD4+ and CD8+ T lymphocytes of each mouse were measured.
RESULTS: The splenic weight of the virus control group is significantly higher than that of the normal control group, with significant splenomegaly. In addition, the splenic inhibition indices of the AZT group and the high- and medium-dose Fuzheng Paidu tablet groups were approximately 93.80%, 37.80%, and 28.07%, respectively. Furthermore, the high and medium dose Fuzheng Paidu tablets could increase the thymus weights of the infected mice.
CONCLUSIONS: Fuzheng Paidu tablets could inhibit splenomegaly, lower the splenic indices, and increase the thymic weights and thymic indices of FLV-induced immunodeficient mice.