这项研究的重点是分离,表征,含量测定,并对麻杏石甘汤(MXSG)不同粒径的胶体颗粒进行抗病毒疗效研究。MXSG的混合胶体相最初被分离成小胶体颗粒段(S),中等胶体颗粒段(M),和大胶体颗粒段(B)使用超滤。使用尺寸排阻色谱法进行进一步的精细分离。采用动态光散射(DLS)和透射电子显微镜(TEM)表征分离的胶体颗粒的尺寸和形态。采用UPLC-MS/MS法测定麻黄碱的含量,苦杏仁苷,甘草酸,采用EDTA络合滴定法测定不同胶体相中的钙(Ca~(2+))含量。最后,通过鼻内给药建立呼吸道合胞病毒(RSV)感染小鼠模型.实验组包括一个空白组,一个模型组,一个利巴韦林组,MXSG组,S组,M组,B组。口服治疗,并评价小鼠肺组织和器官指数的病理变化。研究结果表明,麻黄碱的分布,苦杏仁苷,甘草酸,不同胶体段之间Ca~(2+)含量不均匀。其中,B段在这三种成分中比例最高,除Ca~(2+)外,占46.35%,53.72%,92.36%,分别。尺寸排阻色谱法在100-500nm的尺寸范围内分离出具有均匀形态的胶体颗粒。与S和M段相比,B段显示肺指数抑制率增加(38.31%),脾脏指数,RSV感染小鼠的胸腺指数,改善小鼠肺组织炎症细胞浸润和肺损伤。MXSG中的复杂组分通过加热形成各种尺寸和形态的胶体颗粒,和小分子活性成分如麻黄碱,苦杏仁苷,甘草酸,Ca~(2+)不同程度地参与组装。MXSG抗病毒作用的主要物质基础是在加热过程中由活性组分组装形成的具有一定粒径的胶体颗粒。
This study focused on the separation, characterization, content determination, and antiviral efficacy research on colloidal particles with different sizes in Maxing Shigan Decoction(MXSG). The mixed colloidal phase of MXSG was initially separated into small colloidal particle segment(S), medium colloidal particle segment(M), and big colloidal particle segment(B) using ultrafiltration. Further fine separation was performed using size-exclusion chromatography. Dynamic light scattering(DLS) and transmission electron microscopy(TEM) were employed to characterize the size and morphology of the separated colloidal particles. UPLC-MS/MS was used to determine the content of
ephedrine, amygdalin, glycyrrhizic acid, and the EDTA complexometric titration was used to measure the calcium(Ca~(2+)) content in different colloidal phases. Finally, a respiratory syncytial virus(RSV) infection mouse model was established using intranasal administration. The experimental groups included a blank group, a model group, a ribavirin group, an MXSG group, an S group, an M group, and a B group. Oral administration was given for treatment, and pathological changes in mouse lung tissue and organ indices were evaluated. The results of the study showed that the distribution of
ephedrine, amygdalin, glycyrrhizic acid, and Ca~(2+) content was not uniform among different colloidal segments. Among them, the B segment had the highest proportions of the three components, except for Ca~(2+), accounting for 46.35%, 53.72%, and 92.36%, respectively. Size-exclusion chromatography separated colloidal particles with uniform morphology in the size range of 100-500 nm. Compared to the S and M segments, the B segment showed an increased lung index inhibition rate(38.31%), spleen index, and thymus index in RSV-infected mice, and it improved the infiltration of inflammatory cells and lung injury in the lung tissue of mice. The complex components in MXSG form colloidal particles of various sizes and morphologies through heating, and small-molecule active components such as
ephedrine, amygdalin, glycyrrhizic acid, and Ca~(2+) participate in the assembly to varying degrees. The main material basis for the antiviral effect of MXSG is the colloidal particles with certain particle sizes formed by the assembly of active components during the heating process.