背景:先天性角膜炎(ACC)是一种罕见的遗传性疾病,其特征是人类和动物中皮肤的局部或广泛缺失。患有ACC的人可能会经历骨骼和肌肉系统的发育异常,以及潜在的并发症。局部和孤立的ACC病例可以通过手术和医疗干预进行治疗,而广泛的ACC病例可能导致新生儿死亡。ACC在猪中的存在对动物福利有影响。它导致仔猪出生时死亡率升高,导致生猪养殖业的巨大经济损失。为了阐明与ACC相关的候选遗传基因座,我们对216只杜洛克猪进行了全基因组关联研究分析.这项研究的主要目的是鉴定与ACC相关的候选基因。
结果:本研究确定了9个与ACC相关的显著SNP。进一步分析显示存在两个数量性状基因座,SSC5上的483kb(5:18,196,971-18,680,098)和SSC13上的159kb(13:20,713,440-207294431bp)。通过注释围绕重要SNP的1Mb区域内的候选基因,在SSC5和SSC13上共鉴定出11个候选基因,包括KRT71、KRT1、KRT4、ITGB7、CSAD、RARG,SP7,PFKL,TRPM2、SUMO3和TSPEAR。
结论:这项研究的结果进一步阐明了ACC的潜在机制和遗传结构,并确定了可靠的候选基因。这些结果为治疗和理解人类ACC奠定了基础。
BACKGROUND: Aplasia cutis congenita (ACC) is a rare genetic disorder characterized by the localized or widespread absence of skin in humans and animals. Individuals with ACC may experience developmental abnormalities in the skeletal and muscular systems, as well as potential complications. Localized and isolated cases of ACC can be treated through surgical and medical interventions, while extensive cases of ACC may result in neonatal mortality. The presence of ACC in pigs has implications for animal welfare. It contributes to an elevated mortality rate among piglets at birth, leading to substantial economic losses in the pig farming industry. In order to elucidate candidate genetic loci associated with ACC, we performed a Genome-Wide Association Study analysis on 216 Duroc pigs. The primary goal of this study was to identify candidate genes that associated with ACC.
RESULTS: This study identified nine significant SNPs associated with ACC. Further analysis revealed the presence of two quantitative trait loci, 483 kb (5:18,196,971-18,680,098) on SSC 5 and 159 kb (13:20,713,440-207294431 bp) on SSC13. By annotating candidate genes within a 1 Mb region surrounding the significant SNPs, a total of 11 candidate genes were identified on SSC5 and SSC13, including KRT71, KRT1, KRT4, ITGB7, CSAD, RARG, SP7, PFKL, TRPM2, SUMO3, and TSPEAR.
CONCLUSIONS: The results of this study further elucidate the potential mechanisms underlying and genetic architecture of ACC and identify reliable candidate genes. These results lay the foundation for treating and understanding ACC in humans.