印迹基因在哺乳动物的生长中起着重要作用,发展和行为。Rasgrf1(Ras蛋白特异性鸟嘌呤核苷酸交换因子1)基因已被鉴定为小鼠和大鼠的印迹基因。在本研究中,我们检测到了它的序列,家猪的印记状态和表达模式。在家猪中获得了位于Rasgrf1基因外显子14的228bp部分序列和位于外显子1的193bp部分序列。G/A过渡,在Rasgrf1外显子14中鉴定,然后,采用倒数Berkshire×Wannan黑色F1杂交模型和RT-PCR-RFLP方法检测1日龄发育阶段猪Rasgrf1基因的印迹状态。表达谱结果表明,猪Rasgrf1mRNA在脑中高表达,垂体和胰腺,其次是肾脏,胃,肺,睾丸,小肠,子房,脾脏和肝脏,在背最长肌中表达水平较低,心,和背脂。Rasgrf1基因在脑内的表达水平,两个相互的F1杂种之间的垂体和胰腺组织显着不同。印迹分析表明,猪Rasgrf1基因在肝脏中表达,小肠,父系在肺中表达,但是在大脑中双等位基因表达,心,脾,脾肾,胃,胰腺,背脂,睾丸,子房,背最长肌和垂体组织。
Imprinted genes play important roles in mammalian growth, development and behavior. The Rasgrf1 (Ras protein-specific guanine nucleotide exchange factor 1) gene has been identified as an imprinted gene in mouse and rat. In the present study, we detected its sequence, imprinting status and expression pattern in the domestic pigs. A 228 bp partial sequence located in exon 14 and a 193 bp partial sequence located in exon 1 of the Rasgrf1 gene in domestic pigs were obtained. A G/A transition, was identified in Rasgrf1 exon 14, and then, the reciprocal Berkshire × Wannan black F1 hybrid model and the RT-PCR-RFLP method were used to detect the imprinting status of porcine Rasgrf1 gene at the developmental stage of 1-day-old. The expression profile results indicated that the porcine Rasgrf1 mRNA was highly expressed in brain, pituitary and pancreas, followed by kidney, stomach, lung, testis, small intestine, ovary, spleen and liver, and at low levels of expression in longissimus dorsi, heart, and backfat. The expression levels of Rasgrf1 gene in brain, pituitary and pancreas tissues were significantly different between the two reciprocal F1 hybrids. Imprinting analysis showed that porcine Rasgrf1 gene was maternally expressed in the liver, small intestine, paternally expressed in the lung, but biallelically expressed in brain, heart, spleen, kidney, stomach, pancreas, backfat, testis, ovary, longissimus dorsi and pituitary tissues.