人类二叶虫病是一种广泛的鱼类传播的人畜共患病,是由二叶虫属的广泛tape虫感染引起的。在中国大陆,到目前为止,已经报告了20例人类双叶虫感染病例,根据形态特征或分子分析,将病因学物种鉴定为D.latum和D.nihonkaiense。在本研究中,在黑龙江省发生的3例人类病例中,二叶虫的populottids,通过对线粒体细胞色素c氧化酶亚基I(cox1)和NADH脱氢酶亚基5(nad5)基因进行测序,将中国鉴定为D.nihonkaiense。获得了两种不同的cox1基因序列。一个序列与日本的人类序列显示出100%的同源性。以前没有描述剩余的cox1基因序列和2个不同的nad5基因序列,并可能反映地方性遗传特征。D.nihonkaiense也可能是中国人类双叶病的主要致病物种。同时,在中国发现首例D.nihonkaiense感染的儿科病例表明,感染D.nihonkaiense的婴儿不应被忽视。
Human diphyllobothriasis is a widespread fish-borne zoonosis caused by the infection with broad tapeworms belonging to the genus
Diphyllobothrium. In mainland
China, so far 20 human cases of
Diphyllobothrium infections have been reported, and the etiologic species were identified as D. latum and D. nihonkaiense based on morphological characteristics or molecular analysis. In the present study, proglottids of diphyllobothriid tapeworms from 3 human cases that occurred in Heilongjiang Province,
China were identified as D. nihonkaiense by sequencing mitochondrial cytochrome c oxidase subunit I (cox1) and NADH dehydrogenase subunit 5 (nad5) genes. Two different cox1 gene sequences were obtained. One sequence showed 100% homology with those from humans in Japan. The remaining cox1 gene sequence and 2 different nad5 gene sequences obtained were not described previously, and might reflect endemic genetic characterizations. D. nihonkaiense might also be a major causative species of human diphyllobothriasis in
China. Meanwhile, the finding of the first pediatric case of D. nihonkaiense infection in
China suggests that infants infected with D. nihonkaiense should not be ignored.