B盒蛋白(BBX)编码调节植物昼夜节律和早期光形态发生的锌指转录因子家族。双B盒(DBB)家族属于B盒家族的类,它包含两个保守的B-box结构域,并且缺少一个CCT(CO,CO样和TOC1)图案。在这项研究中,身份,分类,结构,保守的图案,染色体位置,顺式元素,重复事件,并分析了木本模型植物毛果杨中PtrDBB基因的表达谱。这里,确定了12个PtrDBB基因(PtrDBB1-PtrDBB12),并将其分为四个不同的组,它们都均匀分布在十七个杨树染色体中的八个染色体中。毛果假单胞菌和其他两个物种的DBB家族基因的共线性分析(Z。mays和A.thaliana)表明已鉴定出分段重复基因对和高水平保守性。对重复事件的分析证明了对DBB基因进化模式的洞察力。先前发表的转录组数据表明,PtrDBB基因在不同阶段的各种组织中表现出不同的表达模式。此外,据推测,几种PtrDBB参与了对干旱胁迫的响应,亮/暗,ABA和MeJA治疗,这意味着它们可能在非生物胁迫和植物激素反应中起作用。总之,我们的研究结果有助于进一步了解DBB家族,并为毛果假单胞菌PtrDBB基因的潜在功能研究提供参考。
The B-box proteins (BBXs) encode a family of zinc-finger transcription factors that regulate the plant circadian rhythm and early light morphogenesis. The double B-box (DBB) family is in the class of the B-box family, which contains two conserved B-box domains and lacks a CCT (CO, CO-like and TOC1) motif. In this study, the identity, classification, structures, conserved motifs, chromosomal location, cis elements, duplication events, and expression profiles of the PtrDBB genes were analyzed in the woody model plant Populus trichocarpa. Here, 12 PtrDBB genes (PtrDBB1-PtrDBB12) were identified and classified into four distinct groups, and all of them were homogeneously spread among eight out of seventeen poplar chromosomes. The collinearity analysis of the DBB family genes from P. trichocarpa and two other species (Z. mays and A. thaliana) indicated that segmental duplication gene pairs and high-level conservation were identified. The analysis of duplication events demonstrates an insight into the evolutionary patterns of DBB genes. The previously published transcriptome data showed that PtrDBB genes represented distinct expression patterns in various tissues at different stages. In addition, it was speculated that several PtrDBBs are involved in the responsive to drought stress, light/dark, and ABA and MeJA treatments, which implied that they might function in abiotic stress and phytohormone responses. In summary, our results contribute to the further understanding of the DBB family and provide a reference for potential functional studies of PtrDBB genes in P. trichocarpa.