UNASSIGNED:基质辅助激光解吸-电离-飞行时间质谱(MALDI-TOF)最近已广泛用于临床微生物学实验室,具有可靠的优点,快速,和成本效益。这里,我们报告了两种MALDI-TOFMS仪器的性能,EXS3000(Zybio,中国)和Autofms1000(Autobio,中国),是临床微生物学领域常用的。
未经证实:共有209个临床常见分离株,包括70株革兰氏阴性菌,58株革兰氏阳性菌,33个酵母菌株,15株厌氧菌,和33个霉菌菌株,并对19株分枝杆菌进行了检测。所有菌株均通过EXS3000(Zybio,中国)和Autofms1000(Autobio,中国)。使用16SrRNA或ITS区域的序列分析来验证所有菌株。
UNASSIGNED:目前的研究发现,EXS3000和Autofms1000分别发现物种水平的歧视为191(91.39%)和190(90.91%)。EXS3000的属水平区分分别为205(98.09%),Autofms1000的属水平区分为205(98.09%)。对于革兰氏阴性菌,EXS3000物种水平的正确结果为91.43%(64/70),革兰氏阳性球菌占93.1%(54/58),酵母93.94%(31/33),厌氧菌为100%(15/15),丝状真菌为81.82%(27/33)。对于革兰氏阴性菌,Autofms1000的物种水平的正确结果为92.86%(65/70),革兰阳性球菌91.38%(53/58),酵母93.94%(31/33),厌氧菌100%(15/15),丝状真菌78.79%(26/33)。
UNASSIGNED:尽管结果表明,就常规程序的分析效率而言,EXS3000和Autofms1000系统同样是不错的选择,EXS3000的测试结果略优于Autofms1000。值得一提的是,EXS3000仪器的靶板是可重复使用的,但是Autofms1000的目标板是一次性的,使EXS3000更有效地降低成本。
UNASSIGNED: Matrix-assisted laser desorption-ionization-time of flight mass spectrometry (MALDI-TOF) has recently been widely used in clinical microbiology laboratories, with the advantages of being reliable, rapid, and cost-effective. Here, we reported the performance of two MALDI-TOF MS instruments, EXS3000 (Zybio,
China) and Autof ms1000 (Autobio,
China), which are commonly used in clinical microbiology field.
UNASSIGNED: A total of 209 common clinical common isolates, including 70 gram-negative bacteria strains, 58 gram-positive bacteria strains, 33 yeast strains, 15 anaerobic bacteria strains, and 33 mold strains, and 19 mycobacterial strains were tested. All strains were identified by EXS3000 (Zybio,
China) and Autof ms1000 (Autobio,
China). Sequence analysis of 16S rRNA or ITS regions was used to verify all strains.
UNASSIGNED: Current study found that species-level discrimination was found to be 191 (91.39%) and 190 (90.91%) by EXS3000 and Autof ms1000, respectively. Genus-level discrimination was 205 (98.09%) by the EXS3000 and 205 (98.09%) by the Autof ms1000, respectively. The correct results at species level of the EXS3000 were 91.43% (64/70) for gram-negative bacteria, 93.1% (54/58) for gram-positive cocci, 93.94% (31/33) for yeast, 100% (15/15) for anaerobes and 81.82% (27/33) for filamentous fungi. The correct results at species level of the Autof ms1000 were 92.86% (65/70) for gram-negative bacteria, 91.38% (53/58) for gram-positive cocci, 93.94% (31/33) for yeast, 100% (15/15) for anaerobes and 78.79% (26/33) for filamentous fungi.
UNASSIGNED: Although the results show that the EXS3000 and Autof ms1000 systems are equally good choices in terms of analytical efficiency for routine procedures, the test result of EXS3000 is slightly better than Autof ms1000. It\'s worth mentioning that the target plate of the EXS 3000 instrument is reusable, but the target plate of the Autof ms1000 is disposable, making the EXS3000 more effective in reducing costs.