季苯并[c]菲啶生物碱,白屈菜红碱(CHE),具有极大的实践和研究兴趣,广泛的生理效应,主要是抗菌和抗炎,源于它与蛋白质和DNA相互作用的能力。尽管CHE最初被证明具有抗炎特性,其对急性胃溃疡的影响以前尚未被研究过。本研究的目的是评估CHE对乙醇诱导的小鼠胃溃疡的保护作用。在乙醇摄入之前以1、5和10mg/kg体重的剂量施用CHE剂量依赖性地抑制胃溃疡。通过溃疡面积评估胃粘膜病变,胃液酸度,髓过氧化物酶(MPO)活性,宏观和组织病理学检查。CHE显著降低胃溃疡指数,髓过氧化物酶活性,宏观和组织学评分以剂量依赖性方式。此外,CHE还显著抑制一氧化氮(NO)浓度,暴露于乙醇诱导的溃疡小鼠血清和胃粘膜中的促炎性白介素6(IL-6)和肿瘤坏死因子-α(TNF-α)水平呈剂量依赖性。此外,免疫组化分析表明,CHE显着减弱了小鼠胃粘膜中核因子-κB的过度表达。结论是CHE代表了降低胃溃疡风险的潜在治疗选择。此外,急性毒性研究显示用CHE(15mg/kg)治疗的小鼠没有异常体征。这些发现表明,CHE的胃保护活性可能通过调节NF-κB信号通路来调节炎性细胞因子。
The quaternary benzo[c]phenanthridine alkaloid, chelerythrine (
CHE), is of great practical and research interest because of its pronounced, widespread physiological effects, primarily antimicrobial and anti-inflammatory, arising from its ability to interact with proteins and DNA. Although
CHE was originally shown to possess anti-inflammatory properties, its effects on acute gastric ulcer have not been previously explored. The aim of the present study is to evaluate the protective effect of
CHE on ethanol induced gastric ulcer in mice. Administration of CHE at doses of 1, 5 and 10mg/kg bodyweight prior to ethanol ingestion dose-dependently inhibited gastric ulcer. The gastric mucosal lesion was assessed by ulcer area, gastric juice acidity, myeloperoxidase (MPO) activities, macroscopic and histopathological examinations. CHE significantly reduced the gastric ulcer index, myeloperoxidase activities, macroscopic and histological score in a dose-dependent manner. In addition, CHE also significantly inhibited nitric oxide (NO) concentration, pro-inflammatory interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) level in serum and gastric mucosal in the mice exposed to ethanol induced ulceration in a dose-dependent manner. In addition, immunohistochemical analysis revealed that
CHE markedly attenuated the overexpression of nuclear factor-κB in gastric mucosa of mice. It was concluded that CHE represents a potential therapeutic option to reduce the risk of gastric ulceration. In addition, acute toxicity study revealed no abnormal sign to the mice treated with
CHE (15mg/kg). These findings suggest that the gastroprotective activity of CHE might contribute in adjusting the inflammatory cytokine by regulating the NF-κB signalling pathway.