同时含有抗生素抗性细菌(ARB)和抗生素的生物实验室废水是抗生素抗性基因(ARGs)的潜在来源。因此,我们确定了高压灭菌的功效,一种常见的消毒方法,在消除5个ARGs(sul1,sul2,tetW,tetM,amp)和来自实验室废水的整合酶编码基因intI1。高压灭菌(15分钟,121°C)灭活所有细菌,包括ARB,而即使经过60分钟的处理,ARGs仍在废水中保持有限的减少。臭氧化(O3),超声(美国),O3/US,研究了高压灭菌和O3减少实验室废水中ARG的能力。O3和O3/US,所有调查的ARGs的减少率在5.44~7.13log之间.在目前的实验条件下,单独使用US进行废水处理不会减少ARGs(150W,53kHz)。在四种治疗中,高压灭菌后O3处理在最短的时间内显示出最高的还原率;然而,生物实验室废水深度处理需要进一步优化和研究。总的来说,这项研究提供了对ARG来源的新见解,并表明高级氧化方法可用于优化实验室废水处理以进行ARG灭活。实践要点:生物实验室废水是ARGs的潜在水库。常规的高压灭菌不能将ARGs降低到低水平。高压灭菌-O3完全消除所有的细菌。高压灭菌-O3可有效降低ARG(60分钟内去除6.12-7.86对数)。
Biological laboratory wastewater containing both antibiotic-resistant bacteria (ARB) and antibiotics is a potential source of antibiotic resistance genes (ARGs). Thus, we determined the efficacy of
autoclaving, a common disinfection method, in eliminating 5 ARGs (sul1, sul2, tetW, tetM, amp) and the integrase-encoding gene intI1 from laboratory wastewater. Autoclaving (15 min, 121°C) inactivated all bacteria including ARB, whereas ARGs persisted in the wastewater with limited reduction even after 60 min of treatment. Ozonation (O3 ), ultrasound (US), O3 /US, and
autoclaving followed by O3 were investigated for their ability to reduce ARGs in laboratory wastewater. With O3 and O3 /US, the reduction rate ranged from 5.44 to 7.13 log for all ARGs investigated. Wastewater treatment with US alone did not reduce ARGs under the present experimental conditions (150 W, 53 kHz). Among the four treatments, autoclaving followed by O3 treatment showed the highest reduction rates in the shortest time; however, further optimization and investigation are needed for the advanced treatment of bio-laboratory wastewater. Overall, this study provides novel insights into ARG sources and demonstrates that advanced oxidation methods can be useful to optimize laboratory wastewater treatment for ARG inactivation. PRACTITIONER POINTS: Bio-laboratory wastewater is potential reservoir of ARGs. Conventional
autoclaving was not able to reduce ARGs to a low level.
Autoclaving-O3 completely eliminate all the bacteria.
Autoclaving-O3 reduced ARGs efficiently (6.12-7.86 logs removal in 60 min).