背景:蜱和跳蚤被认为是医学和兽医关注的最重要的节肢动物载体,因为它们能够将病原体传播到包括狗在内的一系列动物物种,猫和人类通过与人类分享共同的环境,伴侣动物相关的寄生节肢动物可能会传播人畜共患媒介传播的病原体(VBP)。这项研究旨在从东亚和东南亚的伴侣犬和猫的蜱和跳蚤中分子检测病原体。
方法:从中国的401只动物(即271只狗和130只猫)中收集了392只蜱和248只跳蚤,台湾,印度尼西亚,马来西亚,新加坡,泰国,菲律宾和越南,并对病原体的存在进行分子筛选。测试了立克次体属的蜱虫。,支原体属。,埃里希菌属。,巴贝西亚。和肝菌属。同时对跳蚤进行了立克次体属的筛查。和巴尔通菌属。
结果:在测试的392个蜱中,37(9.4%)对至少一种病原体得分呈阳性,其中肝虫犬是最普遍的(5.4%),其次是犬埃里希氏菌(1.8%),Vogeli巴贝西亚(1%),巴氏无菌血症(0.8%)和立克次体属。(1%)[包括立克次体sp。(0.5%),立克次体(0.3%)和费氏立克次体(0.3%)]。在测试的248只跳蚤中,106例(42.7%)携带至少一种病原体,其中最常见的是R.felis(19.4%),其次是巴尔通菌。(16.5%),立克次体(10.9%)和“立克次体”(0.4%)。此外,对35只血根虫蜱进行了系统发育分析,其中34只蜱属于热带,只有一个属于温带谱系(Rh。sanguineus(sensustricto)。
结论:我们的数据揭示了来自亚洲的狗和猫的蜱和跳蚤中不同VBP的循环,包括人畜共患病原体,这可能对动物和人类健康构成潜在风险。
BACKGROUND: Ticks and fleas are considered amongst the most important arthropod vectors of medical and veterinary concern due to their ability to transmit pathogens to a range of animal species including dogs, cats and humans. By sharing a common environment with humans, companion animal-associated parasitic arthropods may potentially transmit zoonotic vector-borne pathogens (VBPs). This study aimed to molecularly detect pathogens from ticks and fleas from companion dogs and cats in East and Southeast Asia.
METHODS: A total of 392 ticks and 248 fleas were collected from 401 infested animals (i.e. 271 dogs and 130 cats) from
China, Taiwan, Indonesia, Malaysia, Singapore, Thailand, the Philippines and Vietnam, and molecularly screened for the presence of pathogens. Ticks were tested for Rickettsia spp., Anaplasma spp., Ehrlichia spp., Babesia spp. and Hepatozoon spp. while fleas were screened for the presence of Rickettsia spp. and Bartonella spp.
RESULTS: Of the 392 ticks tested, 37 (9.4%) scored positive for at least one pathogen with Hepatozoon canis being the most prevalent (5.4%), followed by Ehrlichia canis (1.8%), Babesia vogeli (1%), Anaplasma platys (0.8%) and Rickettsia spp. (1%) [including Rickettsia sp. (0.5%), Rickettsia asembonensis (0.3%) and Rickettsia felis (0.3%)]. Out of 248 fleas tested, 106 (42.7%) were harboring at least one pathogen with R. felis being the most common (19.4%), followed by Bartonella spp. (16.5%), Rickettsia asembonensis (10.9%) and \"Candidatus Rickettsia senegalensis\" (0.4%). Furthermore, 35 Rhipicephalus sanguineus ticks were subjected to phylogenetic analysis, of which 34 ticks belonged to the tropical and only one belonged to the temperate lineage (Rh. sanguineus (sensu stricto)).
CONCLUSIONS: Our data reveals the circulation of different VBPs in ticks and fleas of dogs and cats from Asia, including zoonotic agents, which may represent a potential risk to animal and human health.