狗钩虫Ancylostomacaninum（Nematoda，Ancylostomatidae）是一种以血液为食的肠道寄生线虫，可引起人类的钩虫病。在这项研究中,通过Illumina深度测序技术对这种人畜共患钩虫的完整线粒体基因组进行了测序。全基因组长度为13,721bp，编码36个基因，包括12个蛋白质编码基因，22个转移RNA，和2个核糖体RNA。系统发育表明，A.caninum与Ancylostoatinae的物种组合在一起，并与Ancylostostomatidae科中的Bunostostoinae物种分开。在Ancylostoatinae亚科中，三只狗起源的A.caninum，不管孤立的起源，聚集在一起，与猫钩虫A.tubaeforme和人类钩虫A.daudenale的关系比狗/猫钩虫A.ceylanicum和海狮钩虫Uncinariasanguinis的关系更密切。一起来看,累积的线粒体DNA数据为线虫的系统发育研究提供了见解。
The dog hookworm Ancylostoma caninum (Nematoda, Ancylostomatidae) is a blood-feeding intestinal parasitic nematode and can cause ancylostomiasis in humans. In this study, the complete mitochondrial genome of this anthropozoonotic hookworm was sequenced through Illumina deep sequencing technology. The whole genome was 13,721 bp in length and encoded 36 genes including 12 protein-coding genes, 22 transfer RNAs, and 2 ribosomal RNAs. Phylogeny revealed that A. caninum grouped with species from Ancylostomatinae and separated from species of Bunostominae in the family Ancylostomatidae. Amongst the subfamily Ancylostomatinae, three dog-originated A. caninum, regardless of isolate origins, clustered together and were more closely related to the cat hookworm A. tubaeforme and the human hookworm A. duodenale than to the dog/cat hookworm A. ceylanicum and the sea lion hookworm Uncinaria sanguinis. Taken together, the cumulative mitochondrial DNA data provides insights into phylogenetic studies among Ancylostomatidae nematodes.
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