金雀花(R.chingii)Hu是其营养质量的关键指标。全面了解R.chingii内的氨基酸代谢有助于该物种衍生的功能性食品的配方和创新。利用先进的技术,如广泛的非靶向代谢组学,转录组分析,交互网络映射,热图分析,和定量实时PCR,我们对R.chingii的四个不同发育阶段的质量属性进行了全面评估。我们细致的分析发现了R.chingii发育阶段的76种不同氨基酸及其衍生物的丰富挂毯。必需氨基酸的谱不仅广泛,而且表现出高度的多样性。值得注意的是,亮氨酸,赖氨酸,苯丙氨酸是最丰富的氨基酸,强调了它们在金吉整个生长周期中的重要存在。在金鸡的整个发育阶段,必需氨基酸相对于总氨基酸含量的比例表现出明显的变化轨迹。它在未成熟的绿色阶段以30.92%开始,在从绿色到黄色的过渡过程中提高到31.04%,在黄色到红色阶段达到33.62%的峰值,然后在全红阶段放缓至30.43%。这种模式表明了氨基酸组成的战略性调节,随着水果的成熟,与不断变化的营养需求和代谢变化保持一致。交互网络和热图的并发分析,除了氨基酸代谢和转录组学检查的全面分析,进行了阐明细胞过程的复杂动力学。结果表明,7个差异表达基因(DEGs)在氨基酸代谢中发挥重要作用,包括PFK,BCAT1,TSB,ASA,ACO,TOM2AH3和BCAT2。七个DEGs的表达模式与先前RNA-seq分析揭示的发现非常吻合。在这次调查中,通过全面的氨基酸谱分析和转录组学分析,我们阐明了在金鸡四个不同发育阶段氨基酸代谢的调控机制。这些见解为利用R.chingii开发新型功能食品应用奠定了基础。
The composition and profile of amino acids in Rubus chingii (R. chingii) Hu serve as critical indicators of its nutritional quality. A comprehensive understanding of the amino acid metabolism within R. chingii is instrumental in the formulation and innovation of functional foods derived from this species. Utilizing advanced techniques such as wide-ranging untargeted metabolomics, transcriptome analysis, interaction network mapping, heat map analysis, and quantitative real-time PCR, we conducted a comprehensive assessment of the quality attributes across four distinct developmental stages of R. chingii. Our meticulous analysis uncovered a rich tapestry of 76 distinct amino acids and their derivatives within the developmental stages of R. chingii. The spectrum of essential amino acids was not only broad but also displayed a high degree of variety. Notably, leucine, lysine, and phenylalanine stood out as the most abundant amino acids, underscoring their significant presence throughout the growth cycle of R. chingii. The proportion of essential amino acids relative to the total amino acid content in R. chingii exhibited a notable trajectory of change throughout its developmental stages. It began with 30.92% in the immature green phase, advanced to 31.04% during the transition from green to yellow, peaked at 33.62% in the yellow to red stage, and then moderated to 30.43% in the full red phase. This pattern suggests a strategic modulation of amino acid composition, aligning with the evolving nutritional requirements and metabolic shifts as the fruit matures. Concurrent analysis of interaction networks and heat maps, alongside comprehensive profiling of amino acid metabolism and transcriptomic examination, was conducted to elucidate the intricate dynamics of cellular processes. The results showed that seven differentially expressed genes (DEGs) played important roles in amino acid metabolism, including PFK, BCAT1, TSB, ASA, ACO, TOM2AH3, and BCAT2. The expression patterns of seven DEGs conformed closely to the findings revealed by the preceding RNA-seq analysis. In this investigation, we elucidated the regulatory mechanisms underlying amino acid metabolism across the four distinct developmental stages of R. chingii through comprehensive amino acid profiling and transcriptomic analysis. These insights lay the groundwork for the development of novel functional food applications utilizing R. chingii.