背景:本研究旨在调查北京女性人群中高危型人乳头瘤病毒(HPV)的流行病学,中国,并确定HPV基因型与宿主因素之间的关系。
方法:于2020年对北京15-89岁(平均年龄38.0±10.9岁)的女性进行HPV检测。高危型HPV基因分型实时聚合酶链反应用于确定HPV基因型。总体患病率,特定年龄的患病率,基因型分布,并分析HPV基因型与宫颈细胞学的相关性。
结果:在25,344名研究参与者中,单、双感染率分别为18.8%(4,777/25,344)和4.2%(1,072/25,344),分别。共有6,119名HPV阳性个体被发现上皮内病变或恶性肿瘤(NILM)的阴性结果为91.6%。5.8%意义不明的非典型鳞状细胞(ASC-US),0.9%低度鳞状上皮内病变(LSIL),和1.7%的高级别鳞状上皮内病变(HSIL)。在单一HPV感染中,HPV16基因型与宫颈细胞学严重程度高度相关(χ2趋势=172.487,P<0.001)。此外,HPV感染率随着年龄的增长而逐渐升高,各年龄组差异有统计学意义(χ2=180.575;P<0.001)。高危HPV基因型在25岁以下的女性和55-59岁的女性中非常普遍。聚类分析显示,在一次感染中,13种HPV基因型可以大致分为两组;然而,未观察到与生物学特征一致的感染模式。
结论:在门诊患者中发现了24.1%的高危型HPV,HPV52、HPV58、HPV16、HPV39和HPV51是最常见的高危基因型。单一高危型HPV感染为主。HPV16、HPV39、HPV51和HPV52与宫颈病变进展相关。HPV16感染尤其令人担忧,因为它加重了宫颈病变。因为13种HPV基因型的感染率因年龄而异,HPV感染率高峰不应指导疫苗接种,筛选,和预防方案。相反,这些举措应根据地区HPV分布特征进行调整.此外,确定北京民众需要接受HPV39感染的治疗。
This study aimed to investigate the epidemiology of high-risk human papillomavirus (HPV) in the female population in Beijing,
China, and identify the relationship between HPV genotypes and host factors.
HPV testing was performed on women aged 15-89 (mean age 38.0 ± 10.9 years) from Beijing in 2020. High-risk HPV genotyping real-time polymerase chain reaction was used to determine HPV genotypes. The overall prevalence, age-specific prevalence, genotype distribution, and the correlation between HPV genotypes and cervical cytology were analyzed.
Among the 25,344 study participants, the single and double infection rates were 18.8% (4,777/25,344) and 4.2% (1,072/25,344), respectively. A total of 6,119 HPV-positive individuals were found to have 91.6% negative results for intraepithelial lesion or malignancy (NILM), 5.8% atypical squamous cells of undetermined significance (ASC-US), 0.9% low-grade squamous intraepithelial lesion (LSIL), and 1.7% high-grade squamous intraepithelial lesion (HSIL). In single HPV infections, the HPV16 genotype was highly associated with cervical cytology severity (χ2 trend = 172.487, P < 0.001). Additionally, HPV infection rates increased gradually with age, and statistical differences were observed across age groups (χ2 = 180.575; P < 0.001). High-risk HPV genotypes were highly prevalent in women below 25 years of age and those aged 55-59 years. Cluster analysis revealed that the 13 HPV genotypes could be roughly divided into two groups in a single infection; however, patterns of infection consistent with biological characteristics were not observed.
High-risk HPV was found in 24.1% of outpatients, with HPV52, HPV58, HPV16, HPV39, and HPV51 being the most common high-risk genotypes. Single high-risk HPV infection was predominant. HPV16, HPV39, HPV51, and HPV52 were associated with cervical lesion progression. HPV16 infection was especially worrying since it aggravates cervical lesions. Because the infection rates of the 13 HPV genotypes differed by age, the peak HPV infection rate should not guide vaccination, screening, and prevention programs. Instead, these initiatives should be tailored based on the regional HPV distribution characteristics. Moreover, it was determined that Beijing\'s populace needed to receive treatment for HPV39 infection.