与丛枝菌根(AM)真菌的共生可改善植物从土壤中的养分捕获,然而,关于多样性的知识有限,结构,功能,和AM真菌相关微生物群落的组装过程。这里,采用16SrRNA基因测序和宏基因组测序技术检测接种和未接种AM真菌的莲花根际细菌,和在南部草地土壤中接种AM真菌的日本L.japonicus突变体ljcbx(共生缺陷)。我们的结果表明,AM共生显著增加了细菌多样性,促进了细菌群落构建的确定性过程,表明菌根共生导致细菌群落的定向富集。AM真菌促进了9种细菌的富集,包括Ohtaekwangia,Niastella,Gemmatatimonas,Devosia,鞘氨醇单胞菌,Novoshingoum,Opitutus,溶菌杆菌,Brevundimonas,与NPK相关参数呈正相关。通过功能识别实验,我们发现其中六个属,包括Brevundimonas,溶菌杆菌,Ohtaekwangia,鞘氨醇单胞菌,Devosia,和Gemmatatimonas,证明了矿化有机磷酸盐和溶解无机磷的能力,氮,钾。我们的研究表明,AM真菌可以调节根际细菌群落的组装,并吸引特定的根际细菌来促进南部草原土壤养分的周转。
Symbiosis with arbuscular mycorrhizal (AM) fungi improves plant nutrient capture from the soil, yet there is limited knowledge about the diversity, structure, functioning, and assembly processes of AM fungi-related microbial communities. Here, 16S rRNA gene sequencing and metagenomic sequencing were used to detect bacteria in the rhizosphere of Lotus japonicus inoculated with and without AM fungi, and the L. japonicus mutant ljcbx (defective in symbiosis) inoculated with AM fungi in southern grassland soil. Our results show that AM symbiosis significantly increased bacterial diversity and promoted deterministic processes of bacterial community construction, suggesting that mycorrhizal symbiosis resulted in the directional enrichment of bacterial communities. AM fungi promoted the enrichment of nine bacteria, including Ohtaekwangia, Niastella, Gemmatimonas, Devosia, Sphingomonas, Novosphingobium, Opitutus, Lysobacter, Brevundimonas, which are positively correlated with NPK-related parameters. Through a functional identification experiment, we found that six of these genera, including Brevundimonas, Lysobacter, Ohtaekwangia, Sphingomonas, Devosia, and Gemmatimonas, demonstrated the ability to mineralize organophosphate and dissolve inorganic phosphorus, nitrogen, and potassium. Our study revealed that AM fungi can regulate rhizosphere bacterial community assembly and attract specific rhizosphere bacteria to promote soil nutrient turnover in southern grasslands.