甲状腺癌是最常见的内分泌癌,甲状腺乳头状癌(PTC)占甲状腺癌的80%-90%。越来越多的研究报道线粒体在调节细胞增殖中起着重要作用。ALDH5A1可能在各种人类癌症中充当癌基因或肿瘤抑制因子,ALDH5A1在PTC中的作用尚不清楚。本研究的目的是探讨ALDH5A1在PTC中的表达及其临床意义。在本研究中,我们在癌症基因组图谱(TCGA)数据库中研究了原发性甲状腺乳头状癌(PTC)中ALDH5A1的表达。结果显示,ALDH5A1水平与肿瘤分期呈正相关,转移,淋巴结分期,和较高水平的ALDH5A1表明无病生存率(DFS)较差。免疫组织化学(IHC)显示,在PTC组织中发现ALDH5A1的表达明显更高。另一方面,ALDH5A1敲低可显著抑制PTC细胞增殖,迁移和侵袭检测发现,ALDH5A1水平降低时,细胞的迁移和侵袭也受到阻碍。ALDH5A1的敲除抑制波形蛋白的表达,促进E-cadherin的表达。简而言之,ALDH5A1的敲低可能作为预防和治疗PTC的新分子靶标。研究意义:本研究集中于ALDH5A1在甲状腺乳头状癌中的作用和潜在机制。我们证明ALDH5A1的表达降低可能通过抑制细胞增殖来抑制TC的进展。迁移和侵袭和逆转上皮间质转化(EMT)。这些发现确保了PTC中ALDH5A1和EMT之间的相互作用关系,为PTC提供了一种新的生物学标记,丰富了TC治疗的潜在策略。
Thyroid cancer is the most common endocrine carcinoma, with papillary thyroid carcinoma (PTC) accounting for 80%-90% of thyroid cancers. Accumulating studies reported that mitochondria plays an important role in the regulation of cell proliferation.
ALDH5A1, may function as an oncogene or tumour suppressor in various human cancers, and the role of
ALDH5A1 in PTC is still unclear. The aim of this study was to investigate the clinical significance of ALDH5A1 expression and its functions in PTC. In this present study, we studied
ALDH5A1 expression on primary papillary thyroid carcinoma (PTC) in The Cancer Genome Atlas (TCGA) database. Results showed that the levels of ALDH5A1 were found positively correlated with tumour stage, metastasis, lymph node stage, and higher levels of
ALDH5A1 demonstrated poor disease-free survival (DFS). Immunohistochemistry (IHC) revealed that significantly higher expression of
ALDH5A1 was found in PTC tissues. On the other hand, knockdown of
ALDH5A1 significantly inhibited PTC cell proliferation, migration and invasion detection found the migration and invasion of cells also were hindered when ALDH5A1 level was reduced. The knockdown of ALDH5A1 inhibited the expression of Vimentin and promoted the expression of E-cadherin. In brief, knockdown of ALDH5A1may act as a novel molecular target for the prevention and treatment of PTC. SIGNIFICANCE OF THE STUDY: The present study focused on the role and the potential mechanism of ALDH5A1 in papillary thyroid carcinoma. We demonstrated that reduced expression of ALDH5A1 might inhibit the progression of TC by inhibiting cell proliferation, migration and invasion and reversing epithelial-mesenchymal transition (EMT). The findings ensured the interaction relation between ALDH5A1 and EMT in PTC, providing a novel biological marker for PTC and enriching the potential strategies for TC treatment.