植酸酶对于增强与植酸结合的磷和其他营养素的生物利用度和释放至关重要,使它们更容易被动物吸收。这项研究是为了检查补充低磷(P)饮食与磷酸二钙(DCP)和液体植酸酶(LP)的效果,其中包含1500FTU/kg,关于增长绩效,肠道形态计量学,接近的身体化学成分,血液剖面,免疫状态,肝脏线粒体酶活性,尼罗罗非鱼的表达响应和经济效益。一式三份鱼组(初始体重5.405±0.045g,N=90)以三种不同的饮食喂养90天。第一种是具有零DCP的对照饮食;第二种是补充有0.71%DCP的对照饮食;第三种是补充有0.03%LP的对照饮食。这些组被指定为CG,DCP和LP,分别。结果表明,LP引起FBW的显着改善(p<0.05),体重增加,增重率,比增长率,HIS,内脏-躯体指数,脾体指数,饲料转化率,血液参数和肠绒毛吸收能力的组织形态计量学评估,与其他组相比。此外,LP显著增加全身蛋白质和脂质含量(p<0.05),与DCP组相比。在复合物I中注意到对植酸酶的阳性反应(p<0.05),线粒体肝复合物酶活性的Ⅲ和Ⅳ。同样,(GHr-1、IGF-1、FAS和LPL)的相对基因表达水平显著(p<0.05)被植酸酶上调,与DCP和对照组相关。Further,植酸酶的总回报和利润百分比最高。可以得出结论,就良好的性能和利润而言,尼罗罗非鱼受益于以0.03%使用植酸酶1500FTU/kg而不添加DCP。
Phytase is crucial in enhancing the bioavailability and release of phosphorus and other nutrients bound to phytic acid, making them more bioavailable for animal absorption. This study was carried out to inspect the effect of supplementing low phosphorus (P) diet with di-calcium phosphate (DCP) and liquid phytase enzyme (LP), which contains 1500 FTU/kg, on growth performance, intestinal morphometry, proximate body chemical composition, blood profile, immunity status, liver mitochondrial enzyme activities, the expression response and economic returns of Nile tilapia (Oreochromis niloticus). Three triplicate groups of fish (initial weight 5.405 ± 0.045 g, N = 90) were fed on three different diets for 90 days. The first was a control diet with zero DCP; the second was a control diet supplemented with 0.71% DCP; the third was a control diet supplemented with 0.03% LP. The groups were designated as CG, DCP and LP, respectively. Results showed that LP induced considerable improvements (p < 0.05) in FBW, body weight gain, weight gain rate, specific growth rate, HIS, viscero-somatic index, spleen-somatic index, feed conversion ratio, blood parameters and the histomorphometry assessment of intestinal villi absorptive capacity, compared with the other groups. Also, whole-body protein and lipid contents pointedly (p < 0.05) increased by LP, compared with the DCP group. A positive response (p < 0.05) to the phytase enzyme was noted in complexes I, III and IV of the mitochondrial liver complex enzyme activity. Likewise, the relative gene expression levels of (GHr-1, IGF-1, FAS and LPL) were notably (p < 0.05) upregulated by phytase enzyme, associated with DCP and control groups. Further, phytase recorded the highest total return and profit percentage. It can be concluded that Nile tilapia benefits from using phytase enzyme 1500 FTU/kg at 0.03% without adding DCP in terms of good performance and profits.