呋喃通过热降解在各种热处理食品中产生,导致男性生殖系统严重受损。本研究的主要目的是研究石榴皮提取物(PGPE)减轻呋喃引起的睾丸功能障碍的潜力。雄性大鼠分为四组:对照/未治疗,PGPE,呋喃,和PGPE+呋喃基团。研究结果表明,呋喃处理的大鼠表现出明显升高的转氨酶和磷酸酶活性,也产生了增加的氧化应激,并降低了抗氧化应激蛋白的活性。此外,蛋白质含量水平(ALT,AST,ALP,和ACP)和类固醇间质细胞羟基类固醇脱氢酶(3β-HSD和17β-HSD)酶的活性显着降低。睾丸参数的显著变化,凋亡基因(Bcl-2,P53和Caspase3),炎症和抗炎细胞因子(IL1β,IL10),男性性激素卵泡刺激素(FSH),黄体生成素(LH),睾丸激素,和精子质量也被观察到。此外,通过生化和分子修饰证实睾丸组织学异常。值得注意的是,与呋喃处理组相比,PGPE预处理的呋喃中毒动物在大多数评估参数中表现出显着改善。总之,PGPE提出了针对呋喃引起的睾丸损伤的基本预防措施和新的潜在药理疗法。
Furan is generated in a wide array of heat-treated foods through thermal degradation, leading to severe impairments in the male reproductive system. The main objective of this study was to investigate the potential of pomegranate peel extract (PGPE) in mitigating testicular dysfunctions induced by furan. Male rats were categorized into four groups: control/untreated, PGPE, furan, and PGPE + furan group. The study results revealed that furan-treated rats exhibited significantly elevated aminotransferase and phosphatase activity, and also generated increased oxidative stress, and reduced antioxidative stress protein activity. Additionally, protein content levels (ALT, AST, ALP, and ACP) and activities of steroidogenic Leydig cell hydroxysteroid dehydrogenase (3β-HSD and 17β-HSD) enzymes were significantly decreased. Significant variations in testicular parameters, apoptotic genes (Bcl-2, P53, and Caspase3), inflammatory and anti-inflammatory cytokines (IL1β, IL10), male sex hormones follicle-stimulating hormone (FSH), luteinizing hormone (LH), testosterone, and sperm quality were also observed. Furthermore, testicular histological abnormalities were confirmed by biochemical and molecular modifications. Notably, PGPE pre-treated furan-intoxicated animals exhibited significant improvements in most of the assessed parameters compared to furan-treated groups. In conclusion, PGPE presents essential preventive measures and a novel pharmacological potential therapy against furan-induced testicular injury.