■头颈部鳞状细胞癌(HNSCC)预后特别差。改善手术切除边界,减少局部复发,最终改善总体生存率是治疗目标。
■要获得完整的手术切除(R0切除),我们研究了靶向整合素亚型αvβ6的荧光成像探针的使用,该亚型在多种上皮癌中上调,使用动物模型。
■使用聚合酶链反应(PCR)和免疫蛋白印迹对人组织进行恶性肿瘤检测αvβ6表达。观察到蛋白质表达定位。αvβ6和表皮生长因子受体(EGFR)通过PCR和免疫蛋白印迹定量,并且使用细胞计数试剂盒-8测定法检查靶向αvβ6探针材料的生物安全性。吲哚菁绿(ICG)用作对照以确定探针在细胞水平上的定位。通过尾静脉注射进行体内动物实验以评估探针的成像效果并确认其在组织切片中的靶向性。
■αvβ6在HNSCC中的表达高于EGFR,并且该探针在体内和体外实验中显示出良好的靶向性,具有良好的安全性。
■ICG-αvβ6肽探针是一种特殊且灵敏的HNSCC成像工具,可以区分肿瘤,正常,和炎症组织。
UNASSIGNED: Head and neck squamous cell carcinoma (HNSCC) has a particularly poor prognosis. Improving the surgical resection boundary, reducing local recurrence, and ultimately ameliorating the overall survival rate are the treatment goals.
UNASSIGNED: To obtain a complete surgical resection (R0 resection), we investigated the use of a fluorescent imaging probe that targets the integrin subtype
αvβ6, which is upregulated in many kinds of epithelial cancer, using animal models.
UNASSIGNED: αvβ6 expression was detected using polymerase chain reaction (PCR) and immunoprotein blotting of human tissues for malignancy. Protein expression localization was observed. αvβ6 and epidermal growth factor receptor (EGFR) were quantified by PCR and immunoprotein blotting, and the biosafety of targeting the
αvβ6 probe material was examined using Cell Counting Kit-8 assays. Indocyanine green (ICG) was used as a control to determine the localization of the probe at the cellular level. In vivo animal experiments were conducted through tail vein injections to evaluate the probe\'s imaging effect and to confirm its targeting in tissue sections.
UNASSIGNED: αvβ6 expression was higher than EGFR expression in HNSCC, and the probe showed good targeting in in vivo and in vitro experiments with a good safety profile.
UNASSIGNED: The ICG-
αvβ6 peptide probe is an exceptional and sensitive imaging tool for HNSCC that can distinguish among tumor, normal, and inflammatory tissues.