新型家畜相关耐甲氧西林金黄色葡萄球菌（MRSA）的检测在临床和家畜中都很重要。在这项研究中,我们报告了一名MRSA感染患者，他与牲畜相关，我们从他们那里收集了两种MRSA菌株FJ0318和FJ0322。为了进一步了解这些MRSA分离株与家畜之间的相关性，对这两个分离株进行了全基因组测序和比较基因组分析.系统发育分析表明这两个菌株是同源的。多位点序列分型显示,这两个菌株均属于ST7,是我国零售肉和肉制品中的常见谱系。FJ0318和FJ0322中的遗传岛与其他常见克隆中的遗传岛不同，例如ST59、ST8和ST5。在菌株FJ0318中发现了具有与来自家畜的质粒pE2的序列相同的序列的镶嵌质粒。此外,在染色体上发现了一个新的预言岛。此外，该岛的序列与在牲畜中鉴定的噬菌体SP6的序列相似。ST7可能来自牲畜并传播到社区，引起侵袭性感染.
The detection of novel livestock-associated methicillin-resistant Staphylococcus aureus (MRSA) is important in both clinics and livestock. In this study, we report a MRSA-infected patient who was associated with livestock as a butcher, from whom we collected two MRSA strains FJ0318 and FJ0322. To further understand the correlation between these MRSA isolates and livestock, whole-genome sequencing and comparative genomic analyses were performed for these two isolates. Phylogenetic analysis revealed that these two strains were homologous. Multilocus sequence typing showed that these two strains belong to ST7, which is a common lineage in retail meat and meat products in China. The genetic islands in FJ0318 and FJ0322 were different from those in other common clones, such as ST59, ST8, and ST5. A mosaic plasmid with a sequence identical to that of the plasmid pE2 from livestock was found in strain FJ0318. Additionally, a novel prophage island was identified on the chromosome. Furthermore, the sequence of the island was similar to that of phage SP6 identified in livestock. ST7 may originate from livestock and be transmitted to communities, causing invasive infections.
PDF(Pubmed)