未经证实:动脉粥样硬化是一种慢性炎症性疾病,仍然是全球发病率和死亡率的主要原因。尽管对这种疾病的发展和进展进行了数十年的研究,目前的管理和治疗方法仍然不能令人满意,需要进一步的研究来了解确切的病理生理学.这篇综述旨在利用单细胞和下一代测序技术对目前发表的数据进行全面评估,以确定动脉粥样硬化和血管炎症的关键细胞和分子贡献。
UASSIGNED:电子搜索Cochrane中央控制试验登记册,MEDLINE,和EMBASE数据库从成立到2022年2月。对所有纳入的研究进行叙述性综合。使用ARRIVE和SYRCLE检查表工具评估质量评估和偏倚风险分析。
未经评估:34项研究符合叙事综合的条件,有16篇文章专门使用单细胞,图10使用下一代测序和8使用这些方法的组合。研究调查了许多目标,从探索性组织和斑块分析,在单细胞和全基因组水平上,细胞表型研究和生理/血液动力学对疾病进展的贡献。一个重要的焦点区域被放在平滑肌细胞上,巨噬细胞,和干细胞/祖细胞对疾病的贡献,很少关注其他细胞类型,包括淋巴细胞和内皮细胞的贡献。相似样品的单细胞测序结果存在显著水平的异质性,导致样本间和研究间的差异。
UNASSIGNED:单细胞和下一代测序方法提供了阐明动脉粥样硬化的新方法,其分辨率明显高于以前的方法。这些方法还显示出可转化为其他血管疾病状态的巨大潜力,通过促进疾病状态之间的细胞特异性基因表达谱。这些技术的实施可以提供新的方法来理解疾病的病理生理学和改善疾病预防。管理,和治疗。系统审查注册:https://www。crd.约克。AC.uk/prospro/display_record.php?ID=CRD42021229960,标识符:CRD42021229960。
UNASSIGNED: Atherosclerosis is a chronic inflammatory disease that remains the leading cause of morbidity and mortality worldwide. Despite decades of research into the development and progression of this disease, current management and treatment approaches remain unsatisfactory and further studies are required to understand the exact pathophysiology. This
review aims to provide a comprehensive assessment of currently published data utilizing single-cell and next-generation sequencing techniques to identify key cellular and molecular contributions to atherosclerosis and vascular inflammation.
UNASSIGNED: Electronic searches of Cochrane Central Register of Controlled Trials, MEDLINE, and EMBASE databases were undertaken from inception until February 2022. A narrative synthesis of all included studies was performed for all included studies. Quality assessment and risk of bias analysis was evaluated using the ARRIVE and SYRCLE checklist tools.
UNASSIGNED: Thirty-four studies were eligible for narrative synthesis, with 16 articles utilizing single-cell exclusively, 10 utilizing next-generation sequencing and 8 using a combination of these approaches. Studies investigated numerous targets, ranging from exploratory tissue and plaque analysis, cell phenotype investigation and physiological/hemodynamic contributions to disease progression at both the single-cell and whole genome level. A significant area of focus was placed on smooth muscle cell, macrophage, and stem/progenitor contributions to disease, with little focus placed on contributions of other cell types including lymphocytes and endothelial cells. A significant level of heterogeneity exists in the outcomes from single-cell sequencing of similar samples, leading to inter-sample and inter-study variation.
UNASSIGNED: Single-cell and next-generation sequencing methodologies offer novel means of elucidating atherosclerosis with significantly higher resolution than previous methodologies. These approaches also show significant potential for translatability into other vascular disease states, by facilitating cell-specific gene expression profiles between disease states. Implementation of these technologies may offer novel approaches to understanding the disease pathophysiology and improving disease prevention, management, and treatment.Systematic
Review Registration: https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42021229960, identifier: CRD42021229960.