背景:低浓度次氯酸(HOCl)漱口水对唾液细菌的影响尚不清楚。我们旨在评估100ppmHOCl漱口水对唾液细菌的抗菌作用,包括金黄色葡萄球菌(S.金黄色葡萄球菌),牙周病(PD)患者。
方法:PD患者随机分为仅漱口水(MW,n=26)和用牙周牙线漱口水(MWPF,n=27)组。选择没有PD的患者作为对照组(n=30)。收集金黄色葡萄球菌培养物和唾液样品(干预之前和之后)用于细菌DNA提取。使用实时聚合酶链反应测定和金黄色葡萄球菌培养物和唾液样品的连续稀释来测量唾液细菌总数(SBTC)并确认使用金黄色葡萄球菌的漱口水的抗菌作用。
结果:三组人口统计学数据无显著差异。在干预之前,MW组和MWPF组的基线SBTC明显高于对照组。漱口水冲洗后,SBTC数据仅在MW和MWPF组中发生显著变化(分别为62.4%和77.4%,分别)。在SBTC数据的base-2对数转换之后,观察到类似的趋势。线性回归显示,基线SBTC和MWPF干预显着影响SBTC体积减少百分比。与10%(v/v)漱口水孵育后,金黄色葡萄球菌106和107个菌落形成单位/mL的存活率分别为0.51%±0.06%和1.42%±0.37%,分别。
结论:这些研究结果表明,100ppmHOCl漱口水可以有效降低PD患者的SBTC和金黄色葡萄球菌的丰度。它提供了HOCl漱口水可以是个人帮助控制SBTC的一种选择,尤其是PD患者。
背景:研究方案于2020年3月20日获得高雄医科大学医院机构审查委员会(KMUHIRB-F(I)-20200042)的批准,并于2022年5月13日在ClinicalTrial.gov(NCT05372835)进行回顾性注册。
The effects of a low concentration of hypochlorous acid (HOCl) mouthwash on salivary bacteria remained unclear. We aimed to evaluate the antibacterial effects of 100 ppm HOCl mouthwash on salivary bacteria, including Staphylococcus aureus (S. aureus), in patients with periodontal disease (PD).
Patients with PD were randomized into mouthwash-only (MW, n = 26) and mouthwash with periodontal flosser (MWPF, n = 27) groups. Patients without PD were selected for the control group (n = 30). S. aureus culture and saliva samples (before and after the intervention) were collected for bacterial DNA extraction. A real-time polymerase chain reaction assay and serial dilutions of S. aureus culture and saliva samples were used to measure the salivary bacteria total count (SBTC) and confirm the antibacterial effects of the mouthwash using S. aureus.
No significant difference in demographic data was observed among the three groups. Before the intervention, the baseline SBTC of the MW and MWPF groups was significantly higher than that of the control group. After the mouthwash rinses, the SBTC data significantly changed in the MW and MWPF groups only (by 62.4% and 77.4%, respectively). After the base-2 log-transformation of the SBTC data, a similar trend was observed. Linear regression revealed that baseline SBTC and the MWPF intervention significantly affected SBTC reduction percentage by volume. After incubation with 10% (v/v) of mouthwash, the survival rates of 106 and 107 colony-forming units/mL of S. aureus were 0.51% ± 0.06% and 1.42% ± 0.37%, respectively.
These
study results indicated that 100 ppm HOCl mouthwash treatment could effectively reduce SBTC in patients with PD and the abundance of S. aureus. It provides that the HOCl mouthwash can be an option for individuals to help control SBTC, especially in patients with PD.
The
study protocol was approved by the Institutional Review Board of Kaohsiung Medical University Hospital (KMUHIRB-F(I)-20200042) on 20/03/2020 and retrospectively registered at ClinicalTrial.gov (NCT05372835) on 13/05/2022.