方法:腹泻是一种常见的健康问题,导致全球儿童和老年人的年死亡率高。鼠李糖乳杆菌GG(LGG)和单宁酸(TA)的抗腹泻活性,单独或组合,被检查,除了它们对肠屏障完整性的影响。
结果:将56只成年雄性Wistar大鼠随机分为7组:对照组,只有LGG,TA独自一人,腹泻模型,腹泻+LGG,腹泻+TA,和腹泻+LGG+TA治疗组。腹泻是由高乳糖饮食(HLD)引起的。在HLD喂养后4天口服LGG(1×109CFU/大鼠)和TA(100mgKg-1d-1),并持续10天。回肠标本被处理用于局部肠道细胞因子的生化分析,聚合酶链反应(PCR),和组织学研究。此外,进行基于免疫组织化学的增殖细胞核抗原(PCNA)和闭塞带1(ZO-1)的鉴定。与腹泻模型组相比,两种治疗方法都能维持肠粘膜结构和增殖活性,并保持ZO-1的表达,组合组显示最大效果。然而,LGG治疗的腹泻大鼠肠组织中肿瘤坏死因子-α(TNF-α)和核因子-κB(NF-κB)的浓度明显降低;TA处理导致干扰素-γ(INF-γ)和转化生长因子-β(TGF-β1)的选择性降低。
结论:个别LGG和TA治疗可显著缓解腹泻,可能是通过选择性免疫调节细胞因子依赖性机制,而两者的结合通过保持肠上皮屏障功能和再生能力来协同维持肠粘膜。
METHODS: Diarrhea is a common health issue that contributes to a significant annual death rate among children and the elderly worldwide. The anti-diarrheal activity of Lactobacillus rhamnosus GG (LGG) and tannic acid (TA), alone or combined, is examined, in addition to their effect on intestinal barrier integrity.
RESULTS: Fifty-six adult male Wistar rats are randomly assigned into seven groups: control, LGG alone, TA alone, diarrhea model, diarrhea+LGG, diarrhea+TA, and diarrhea+LGG+TA-treated groups. Diarrhea is induced by high-lactose diet (HLD) consumption. LGG (1x109 CFU/rat) and TA (100 mg Kg-1 d-1) were given orally 4 days after HLD feeding and continued for 10 days. Ileum specimens are processed for biochemical analysis of the local intestinal cytokines, polymerase chain reaction (PCR), and histological study. Also, immunohistochemistry-based identification of Proliferating Cell Nuclear Antigen (PCNA) and zonula occludens 1 (ZO-1) is performed. Compared to the diarrhea model group, both treatments maintain the intestinal mucosal structure and proliferative activity and preserve ZO-1 expression, with the combination group showing the maximal effect. However, LGG-treated diarrheic rats show a remarkable decrease in the intestinal tissue concentrations of tumor necrosis factor-alpha (TNF-α) and nuclear factor Kappa beta (NF-κB); meanwhile, TA treatment leads to a selective decrease of interferon-gamma (INF-γ) and transforming growth factor-beta (TGF-β1).
CONCLUSIONS: Individual LGG and TA treatments significantly alleviate diarrhea, probably through a selective immunomodulatory cytokine-dependent mechanism, while the combination of both synergistically maintains the intestinal mucosa by keeping the intestinal epithelial barrier function and regenerative capability.