这篇文献综述将提供关于所报道的角膜缘干细胞缺乏症(LSCD)动物模型的亮点和潜在陷阱的关键叙述概述,并将确定该研究领域被忽视的方面。在文献中存在关于用于创建模型的方法和当模型被认为完全适合用于评估和/或用于测试各种治疗干预时的损伤后的预定义持续时间的显著异质性。文献中也有许多实例,其中特定模型的实施在不同研究中显著不同。例如,化学物质的浓度,以及其在化学诱导的LSCD模型中的暴露时间和技术,不仅对模型的有效性有很大的影响,而且对并发症的严重程度也有很大的影响。此外,虽然一些模型诱发了全面的LSCD临床表现,有些是阻碍他们的能力只产生部分LSCD。要考虑的另一个方面是由特定方法引起的损坏的性质。由于热方法会导致更多的基质疤痕,它们可能更适合评估特定治疗的抗纤维化特性。另一方面,因为化学烧伤会导致更多的血管新生,它们提供了利用抗新生血管形成的潜在治疗方法的机会。动物物种(即,老鼠,老鼠,兔子,等。)也是模型有效性及其临床翻译潜力的关键因素,每种动物都有其独特的优点和缺点。这篇综述还将阐述其他被忽视的方面,例如实验期间使用的麻醉剂,动物的性别,LSCD诱导后的护理,和模型验证。审查将通过提供未来的观点和建议在这个相当重要的研究领域的进一步发展。
This literature
review will provide a critical narrative overview of the highlights and potential pitfalls of the reported animal models for limbal stem cell deficiency (LSCD) and will identify the neglected aspects of this research area. There exists significant heterogeneity in the literature regarding the methodology used to create the model and the predefined duration after the insult when the model is supposedly fully fit for evaluations and/or for testing various therapeutic interventions. The literature is also replete with examples wherein the implementation of a specific model varies significantly across different studies. For example, the concentration of the chemical, as well as its duration and technique of exposure in a chemically induced LSCD model, has a great impact not only on the validity of the model but also on the severity of the complications. Furthermore, while some models induce a full-blown clinical picture of total LSCD, some are hindered by their ability to yield only partial LSCD. Another aspect to consider is the nature of the damage induced by a specific method. As thermal methods cause more stromal scarring, they may be better suited for assessing the anti-fibrotic properties of a particular treatment. On the other hand, since chemical burns cause more neovascularisation, they provide the opportunity to tap into the potential treatments for anti-neovascularisation. The animal species (i.e., rats, mice, rabbits, etc.) is also a crucial factor in the validity of the model and its potential for clinical translation, with each animal having its unique set of advantages and disadvantages. This
review will also elaborate on other overlooked aspects, such as the anaesthetic(s) used during experiments, the gender of the animals, care after LSCD induction, and model validation. The
review will conclude by providing future perspectives and suggestions for further developments in this rather important area of research.