目的:虽然5%聚维酮碘(PVP-I)经常用作眼部防腐剂,关于PVP-I浓度的影响缺乏共识,打开后的储存,和复合制剂对PVP-I的抗菌作用。我们进行了一系列体外实验,以确定这些因素对PVP-I抑制术后眼部感染常见原因的影响。
方法:在体外测量作为活性PVP-I暴露时间的函数的微生物生长的抑制。在对照实验中,PVP-I在微生物暴露之前被灭活。测试的PVP-I溶液浓度不同(0.6%,5%,或10%),打开后的储存时间(0、7或30天),和制备(商业与从储备PI溶液复合)。测试的病原体包括表皮葡萄球菌,S.viridans,铜绿假单胞菌,耐甲氧西林金黄色葡萄球菌,甲氧西林敏感的金黄色葡萄球菌,还有白色念珠菌.
结果:PVP-I溶液抑制所有细菌生长3分钟,抑制真菌生长15秒。与5%PVP-I相比,0.6%的PVP-I对抑制绿脓杆菌生长的效果较差(200±0个菌落与30秒时7±8,P=0.0004;183±21vs.1分钟时0±0,P=0.018),但更有效地抑制铜绿假单胞菌(30±20vs.在15秒时200±0,P=0.019)。与商业和新开放的PVP-I解决方案相比,复合制剂和溶液在开瓶后储存7或30天,保存或改善对测试微生物的防腐功效。
结论:PVP-I溶液的浓度影响暴露后1分钟内的防腐效果,但所有的解决方案在3分钟内等效地执行。与先前研究稀释PVP-I的结果相反,0.6%PVP-I没有表现出一致等效或优异的抗菌作用.开瓶后的复合制备和储存长度没有降低PVP-I防腐活性。
OBJECTIVE: Although 5% povidone-iodine (PVP-I) is frequently used as an ocular antiseptic agent, there is a lack of consensus regarding the effects of PVP-I concentration,
storage after opening, and compounded preparation on PVP-I antisepsis. We performed a series of in-vitro experiments to determine the impact of these factors on PVP-I\'s inhibition of common causes of post-procedural eye infection.
METHODS: Inhibition of microorganism growth was measured in-vitro as a function of active PVP-I exposure time. In control experiments, PVP-I was inactivated before microorganism exposure. Tested PVP-I solutions varied in concentration (0.6%, 5%, or 10%), length of
storage after opening (0, 7, or 30 days), and preparation (commercial vs.compounded from stock PI solution). Tested pathogens included S. epidermidis, S. viridans, P. aeruginosa, methicillin-resistant S. aureus, methicillin-sensitive S. aureus, and C. albicans.
RESULTS: PVP-I solutions inhibited all bacterial growth by 3 min and fungal growth by 15 s. Compared to 5% PVP-I, the 0.6% PVP-I was less effective in inhibiting S. viridans growth (200 ± 0 colonies vs. 7 ± 8 at 30 s, P = 0.0004; 183 ± 21 vs. 0 ± 0 at 1 min, P = 0.018), but more effective in inhibiting P. aeruginosa (30 ± 20 vs. 200 ± 0 at 15 s, P = 0.019). Compared to commercial and newly-opened PVP-I solutions, compounded preparations and solutions stored for 7 or 30 days after bottle opening either preserved or improved antiseptic efficacy against tested microorganisms.
CONCLUSIONS: Concentration of PVP-I solution affects antiseptic efficacy within 1 min of exposure, but all solutions performed equivalently at 3 min. In contrast to results of prior studies investigating dilute PVP-I, the 0.6% PVP-I did not demonstrate a uniformly equivalent or superior anti-septic effect. Compounded preparation and
storage length after bottle opening did not decrease PVP-I antiseptic activity.