曼陀罗metelL.(刺苹果)已在泰国民间智慧中用于伤口护理。在这项研究中,我们选择了超临界二氧化碳萃取(scCO2)来从刺苹果的叶子中进行粗萃取。使用液相色谱-四极杆飞行时间质谱(LC-QTOF-MS)观察植物化学谱。D.metel的生物活性是通过抗氧化剂测定进行的,基于格里斯反应的抗炎,迁移试验,基质金属蛋白酶-2(MMP-2)的表达,和成纤维细胞中的调节基因。在300和500巴的不同压力下,从scCO2程序中获得Dm1和Dm2提取物,分别。生物活性化合物,包括法尼基丙酮,五苯甲酚B,和Loliolide,在两种提取物中都得到了鉴定。两种D.metel提取物的抗氧化特性与过氧化氢诱导的成纤维细胞中的l-抗坏血酸相当,没有显着差异。此外,Dm1和Dm2显著抑制亚硝酸盐产生水平为1.23±0.19和1.52±0.05μM,分别,对脂多糖处理组(3.82±0.39μM)。有趣的是,Dm1在治疗36和48h后明显显示伤口闭合的百分比为58.46±7.61和82.62±6.66%,与来自小牛血提取物的商业脱蛋白透析液相当。此外,两种提取物在抑制MMP-2表达的能力方面与l-抗坏血酸处理相当:MMP-2是一种分解胶原蛋白的酶.SHH的基因表达,SMO,Dm1也明显上调了控制Sonichedgehog途径的GLI1。因此,scCO2技术可以应用于D.metel提取,并有助于潜在的有效伤口闭合。
Datura metel L. (thorn apple) has been used in Thai folk wisdom for wound care. In this
study, we chose supercritical carbon dioxide extraction (scCO2) to develop crude extraction from the leaves of the thorn apple. The phytochemical profiles were observed using liquid chromatography-quadrupole time-of-flight mass spectrometry (LC-QTOF-MS). The biological activities of D. metel were performed through antioxidant assays, anti-inflammation based on the Griess reaction, the migration assay, the expression of matrix metalloproteinase-2 (MMP-2), and regulatory genes in fibroblasts. Dm1 and Dm2 extracts were obtained from scCO2 procedures at different pressures of 300 and 500 bar, respectively. Bioactive compounds, including farnesyl acetone, schisanhenol B, and loliolide, were identified in both extracts. The antioxidant properties of both D. metel extracts were comparable to those of l-ascorbic acid in hydrogen peroxide-induced fibroblasts with no significant difference. Additionally, Dm1 and Dm2 significantly inhibited the nitrite production levels of 1.23 ± 0.19 and 1.52 ± 0.05 μM, respectively, against the lipopolysaccharide-treated group (3.82 ± 0.39 μM). Interestingly, Dm1 obviously demonstrated the percentage of wound closure with 58.46 ± 7.61 and 82.62 ± 6.66% after 36 and 48 h of treatment, which were comparable to the commercial deproteinized dialysate from the calf blood extract. Moreover, both extracts were comparable to l-ascorbic acid treatment in their ability to suppress the expression of MMP-2: an enzyme that breaks down collagen. The gene expressions of SHH, SMO, and GLI1 that control the sonic hedgehog pathway were also clearly upregulated by Dm1. Consequently, the scCO2 technique could be applied in D. metel extraction and contribute to potentially effective wound closure.