单细胞(sc)转录组学的最新进展彻底改变了我们对树突状细胞(DC)的理解。免疫系统的关键角色。ScRNA测序（scRNA-seq）揭示了以前无法识别的DC亚群的复杂性和异质性，揭示他们的个体发育和专业角色。然而,对于不熟悉该领域的研究人员来说，导航快速的技术进步和计算方法可能是令人生畏的。这篇综述旨在为免疫学家提供sc转录组学分析的全面介绍，提供对DC生物学最新发展的见解。解决常见的分析查询，我们引导读者通过流行的工具和方法，补充参考基准和教程，以深入了解。通过检查开创性研究的发现，我们说明了计算技术如何扩展了我们对DC生物学的认识。通过这种合成，我们的目标是为研究人员提供必要的工具和知识，以导航和利用scRNA-seq来解开DC生物学的复杂性并推进免疫学研究。
Recent advances in single-cell (sc) transcriptomics have revolutionized our understanding of dendritic cells (DCs), pivotal players of the immune system. ScRNA-sequencing (scRNA-seq) has unraveled a previously unrecognized complexity and heterogeneity of DC subsets, shedding light on their ontogeny and specialized roles. However, navigating the rapid technological progress and computational methods can be daunting for researchers unfamiliar with the field. This review aims to provide immunologists with a comprehensive introduction to sc transcriptomic analysis, offering insights into recent developments in DC biology. Addressing common analytical queries, we guide readers through popular tools and methodologies, supplemented with references to benchmarks and tutorials for in-depth understanding. By examining findings from pioneering studies, we illustrate how computational techniques have expanded our knowledge of DC biology. Through this synthesis, we aim to equip researchers with the necessary tools and knowledge to navigate and leverage scRNA-seq for unraveling the intricacies of DC biology and advancing immunological research.
