背景:蓝细菌是具有广泛形态和代谢多样性的原核生物。通过光合作用,它们将无机化合物转化为生物分子,可能有商业利益。在这项工作中,我们评估了20个蓝细菌菌株的生理方面,如生长,光合作用和生化成分,其中一些是首次在这里披露。将生物在圆柱形光生物反应器(CPBR)中培养144小时,并获得生物量。培养物内部的光在整个实验时间内是恒定的,并保持在每个物种的饱和辐照度(Ik)下。通过自动CO2鼓泡将培养物的pH维持在7.8和8.4内。增长率,干生物量,叶绿素a,类胡萝卜素,藻蓝蛋白,蛋白质,碳水化合物,脂质,聚羟基链烷酸酯(PHA)和抗氧化活性测定。
结果:生化成分的比例因物种而异,以及增长率。Leptolyngbyasp.和Nostocsp。(CCIBt3249)的增长率在0.7-0.8d-1的范围内,其次是Rhabdordermasp。(~0.6d-1),和Phormidiumsp.(~0.5d-1)。在横纹肌中获得了高类胡萝卜素含量。(4.0μgmL-1)和藻蓝蛋白。(60μgmL-1)。在Geitlerinema属(75%DW)中发现了更高的总蛋白,纳瓦氏微囊藻的碳水化合物(30%DW)和Phormidiumsp。的脂质。(15%DW)。此外,Aphanocapsaholsatica显示出最高的抗氧化活性(65%)和巴西Sphaerocavum,铜绿微囊藻,Nostocsp.(CCIBt3249)和A。holsatica较高水平的PHA(〜2%DW)。
结论:这项研究报告了蓝藻的生化成分,这些成分可以影响其生产的生物技术,突出具有特定生物分子高生产率的潜在菌株。
BACKGROUND: Cyanobacteria are prokaryotic organisms with wide morphological and metabolic diversity. By means of photosynthesis, they convert inorganic compounds into biomolecules, which may have commercial interest. In this work, we evaluated 20 cyanobacterial strains regarding their physiological aspects such as growth, photosynthesis and biochemical composition, some of which are revealed here for the first time. The organisms were cultivated in cylindrical photobioreactors (CPBR) for 144 h and the biomass was obtained. The light inside cultures was constant throughout experimental time and maintained at the saturation irradiance (Ik) of each species. Culture pH was maintained within 7.8 and 8.4 by automatic CO2 bubbling. Growth rate, dry biomass, chlorophyll a, carotenoids, phycocyanin, proteins, carbohydrates, lipids, polyhydroxyalkanoate (PHA) and antioxidant activity were determined.
RESULTS: The proportionality of the biochemical composition varied among species, as well as the growth rates. Leptolyngbya sp. and Nostoc sp. (CCIBt3249) showed growth rates in the range of 0.7-0.8 d-1, followed by Rhabdorderma sp. (~ 0.6 d-1), and Phormidium sp. (~ 0.5 d-1). High carotenoid content was obtained in Rhabdoderma sp. (4.0 μg mL-1) and phycocyanin in Leptolyngbya sp. (60 μg mL-1). Higher total proteins were found in the genus Geitlerinema (75% DW), carbohydrates in Microcystis navacekii (30% DW) and lipids in Phormidium sp. (15% DW). Furthermore, Aphanocapsa holsatica showed the highest antioxidant activity (65%) and Sphaerocavum brasiliense, Microcystis aeruginosa, Nostoc sp. (CCIBt3249) and A. holsatica higher levels of PHA (~ 2% DW).
CONCLUSIONS: This
study reports on the biochemical composition of cyanobacteria that can impact the biotechnology of their production, highlighting potential strains with high productivity of specific biomolecules.