■Rumexvesicarius是一种野生叶类植物,属于Polygoneae科,以其治疗益处而闻名。Rumex属包括全球分布的约150种。
■该研究旨在使用体外和计算机模拟方法研究膀胱R的生物学活性。
■从冰雹的山上收集Rumexvesicarius,并用甲醇提取。使用比色检测方法定性地确定植物化学成分。其他分析包括元素分析,在硅对接中,抗氧化剂,抗菌,和抗生物膜特性。
■提取物含有各类植物化学物质,包括类黄酮,酚类物质,单宁,萜烯,和皂苷。通过分子对接鉴定了16种成分,显示对丝状温度敏感蛋白Z(FtsZ)的抑制作用,细菌细胞分裂的关键因素。六种化合物表现出-8.3至-5.0kcal/mol的低结合评分,表明在活性位点的有效相互作用。元素分析确定了15个元素,钾是最丰富的,其次是钙,铝,硅,铁,磷,硫磺,镁,钛,锶,锌,锰,溴,和铬。抗氧化剂分析显示,与抗坏血酸相比,在较低浓度下具有显着的特性,丁基化羟基甲苯,和β-胡萝卜素.抗菌分析显示对枯草芽孢杆菌MTCC121和铜绿假单胞菌MTCC741有抑制作用,抑制区分别为13.67±1.0mm和11.50±1.0mm,分别。MIC和MBC值范围为250至500μg/mL。膀胱R.也表现出抗生物膜活性。
■来自冰雹山脉的野生R.vesicarius富含生物活性植物化学物质和必需矿物质,表现出显著的抗氧化和抗菌性能。
UNASSIGNED: Rumex vesicarius is a wild leafy plant belonging to the family Polygonaceae, renowned for its therapeutic benefits. The genus Rumex comprises approximately 150 species distributed globally.
UNASSIGNED: The
study aimed to investigate the biological activities of R. vesicarius using in vitro and in silico methods.
UNASSIGNED: Rumex vesicarius was collected from the mountains in Hail and extracted with methanol. The phytochemical composition was qualitatively determined using colorimetric detection methods. Additional analyses included elemental analysis, in silico docking, antioxidant, antibacterial, and anti-biofilm properties.
UNASSIGNED: The extract contained various classes of phytochemicals, including flavonoids, phenolics, tannins, terpenes, and saponins. Sixteen constituents were identified through molecular docking, revealing inhibition against the filamentous temperature-sensitive protein Z (FtsZ), a crucial factor in bacterial cell division. Six compounds exhibited low binding scores ranging from -8.3 to -5.0 kcal/mol, indicating efficient interaction at the active site. Elemental analysis identified 15 elements, with potassium being the most abundant, followed by calcium, aluminum, silicon, iron, phosphorus, sulfur, magnesium, titanium, strontium, zinc, manganese, bromine, and chromium. Antioxidant analysis revealed significant properties at lower concentrations compared to ascorbic acid, butylated hydroxytoluene, and β-carotene. Antibacterial analysis demonstrated inhibitory effects on Bacillus subtilis MTCC121 and Pseudomonas aeruginosa MTCC 741, with inhibition zones of 13.67 ± 1.0 mm and 11.50 ± 1.0 mm, respectively. The MIC and MBC values ranged from 250 to 500 μg/mL. R. vesicarius also exhibited anti-biofilm activity.
UNASSIGNED: Wild-grown R. vesicarius from the mountains of Hail is rich in bioactive phytochemicals and essential minerals, exhibiting notable antioxidant and antibacterial properties.