Photosynthesis in vegetation is one of the key processes in maintaining regional ecological balance and climate stability, and it is of significant importance for understanding the health of regional ecosystems and addressing climate change. Based on 2001-2021 Global OCO-2 Solar-Induced Fluorescence (GOSIF) dataset, this study analyzed spatiotemporal variations in Asian vegetation photosynthesis and its response to climate and human activities. Results show the following: (1) From 2001 to 2021, the overall photosynthetic activity of vegetation in the Asian region has shown an upward trend, exhibiting a stable distribution pattern with higher values in the eastern and southern regions and lower values in the central, western, and northern regions. In specific regions such as the Turgen Plateau in northwestern Kazakhstan, Cambodia, Laos, and northeastern Syria, photosynthesis significantly declined. (2) Meteorological factors influencing photosynthesis exhibit differences based on latitude and vertical zones. In low-latitude regions, temperature is the primary driver, while in mid-latitude areas, solar radiation and precipitation are crucial. High-latitude regions are primarily influenced by temperature, and high-altitude areas depend on precipitation and solar radiation. (3) Human activities (56.44%) have a slightly greater impact on the dynamics of Asian vegetation photosynthesis compared to climate change (43.56%). This research deepens our comprehension of the mechanisms behind the fluctuations in Asian vegetation photosynthesis, offering valuable perspectives for initiatives in environmental conservation, sustainability, and climate research.

OBJECTIVE: Niche differentiation is a crucial issue in speciation. Although it has a well-known role in adaptive processes of hybrid angiosperms, it is less understood in hybrid ferns. Here, we investigate whether an intermediate ecological niche of a fern hybrid is a novel adaptation that provides insights into fern hybrid speciation.
METHODS: Pteris fauriei (Pteridaceae) is a natural hybrid fern, occurring in environments between its parent species. The maternal Pteris minor is found in sunny areas, but the habitat of the paternal Pteris latipinna is shady. We combined data from morphology, leaf anatomy and photosynthetic traits to explore adaptation and differentiation, along with measuring the environmental features of their niches. We also performed experiments in a common garden to understand ecological plasticity.
RESULTS: The hybrid P. fauriei was intermediate between the parent species in stomatal density, leaf anatomical features and photosynthetic characteristics in both natural habitats and a common garden. Interestingly, the maternal P. minor showed significant environmental plasticity and was more similar to the hybrid P. fauriei in the common garden, suggesting that the maternal species experiences stress in its natural habitats but thrives in environments similar to those of the hybrid.
CONCLUSIONS: Based on the similar niche preferences of the hybrid and parents, we propose hybrid superiority. Our results indicate that the hybrid P. fauriei exhibits greater fitness and can compete with and occupy the initial niches of the maternal P. minor. Consequently, we suggest that the maternal P. minor has experienced a niche shift, elucidating the pattern of niche differentiation in this hybrid group. These findings offer a potential explanation for the frequent occurrence of hybridization in ferns and provide new insights into fern hybrid speciation, enhancing our understanding of fern diversity.

We hypothesized that anthocyanins act as a sugar-buffer and an alternative electron sink during leaf senescence to prevent sugar-mediated early senescence and photoinhibition. To elucidate the role of anthocyanin, we monitored seasonal changes in photosynthetic traits, sugar, starch and N contents, pigment composition, and gene expression profiles in leaves exposed to substantially different light conditions within a canopy of an adult fullmoon maple (Acer japonicum) tree. Enhancement of starch amylolysis accompanied by cessation of starch synthesis occurred in the same manner independent of light conditions. Leaf sugar contents increased, but reached upper limits in the late stage of leaf senescence, even though leaf anthocyanins further increased after complete depletion of starch. Sun-exposed leaves maintained higher energy consumption via electron flow than shade-grown leaves during leaf N resorption. Thus, anthocyanins accumulated in sun-exposed leaves might have a regulative role as a sugar-buffer, retarding leaf senescence, and an indirect photoprotective role as an alternative sink for electron consumption to compensate declines in other metabolic processes such as starch and protein synthesis. In this context, anthocyanins may be key substrates protecting both outer-canopy leaves (against photoinhibition) and inner-canopy leaves (via shading by outer-canopy leaves) from high light stress during N resorption.

Leaf gas exchange measurements are an important tool for inferring a plant\'s photosynthetic biochemistry. In most cases, the responses of photosynthetic CO2 assimilation to variable intercellular CO2 concentrations (A/Ci response curves) are used to model the maximum (potential) rate of carboxylation by ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco, Vcmax) and the rate of photosynthetic electron transport at a given incident photosynthetically active radiation flux density (PAR; JPAR). The standard Farquhar-von Caemmerer-Berry model is often used with default parameters of Rubisco kinetic values and mesophyll conductance to CO2 (gm) derived from tobacco that may be inapplicable across species. To study the significance of using such parameters for other species, here we measured the temperature responses of key in vitro Rubisco catalytic properties and gm in cotton (Gossypium hirsutum cv. Sicot 71) and derived Vcmax and J2000 (JPAR at 2000 µmol m-2 s-1 PAR) from cotton A/Ci curves incrementally measured at 15°C-40°C using cotton and other species-specific sets of input parameters with our new automated fitting R package \'OptiFitACi\'. Notably, parameterisation by a set of tobacco parameters produced unrealistic J2000:Vcmax ratio of <1 at 25°C, two- to three-fold higher estimates of Vcmax above 15°C, up to 2.3-fold higher estimates of J2000 and more variable estimates of Vcmax and J2000, for our cotton data compared to model parameterisation with cotton-derived values. We determined that errors arise when using a gm,25 of 2.3 mol m-2 s-1 MPa-1 or less and Rubisco CO2-affinities in 21% O2 (KC 21%O2) at 25°C outside the range of 46-63 Pa to model A/Ci responses in cotton. We show how the A/Ci modelling capabilities of \'OptiFitACi\' serves as a robust, user-friendly, and flexible extension of \'plantecophys\' by providing simplified temperature-sensitivity and species-specificity parameterisation capabilities to reduce variability when modelling Vcmax and J2000.

Remote sensing of Solar-induced chlorophyll fluorescence (SIF) has been widely used in estimating Gross Primary Productivity (GPP) and detecting stress in terrestrial ecosystems. Water stress adversely impacts the growth, development, and productivity of a plant. Recently, the characterizing and understanding of the diurnal cycling of plant functioning and ecosystem processes has been explored using SIF. However, the diurnal response of SIF to different levels of water stress remains unclear. This study conducted field experiments on winter wheat by subjecting it to different levels of water stress including well-watered (CK) and, mild, moderate, and severe water stress (D1, D2, D3), and collected the spectral data using an automated SIF measurement system. The results observed the strong SIF-PAR (photosynthetically active radiation) correlations and that these relationships gradually decoupled with increasing water stress, which further decreased the accuracy of temporal upscaling of far-red SIF from an instantaneous to daily scale. To quantify the characteristics of diurnal far-red SIF, five indices including peak time, peak value, curve opening coefficient (leading coefficient of the parabola), and left/right slopes of the peak were proposed. The results demonstrated that diurnal far-red SIF was characterized by an earlier peak time, decreasing peak value, wider curve opening, and flattening right slope from the CK plot to the D3 plot. There were certain mechanisms linking the different indices, for example, between peak size and opening coefficient. Furthermore, the response of far-red SIF to water stress was most pronounced at noon. SIF/PAR exhibited a more significant response to varying water stress compared to far-red SIF, which mitigated the negative influence of PAR variations on diurnal SIF. These findings contribute to the monitoring of plant water dynamics at fine temporal scales.

Wide availability of anthropogenic TiO2 nanoparticles facilitates their penetration into environment and prompts interactions with plants. They alter plants growth and change their nutritional status. In particular, metabolic processes are affected. In this work the effect of nanometric TiO2 on photosynthesis efficiency in green pea (Pisum sativum L.) was studied. Hydroponic cultivations with three Ti levels (10; 50 and 100 mg L-1) were applied. At all concentrations nanoparticles penetrated into plant tissues and were detected by the single particle ICP-MS/MS method. Nanoparticles altered the CO2 assimilation rate and gas exchange parameters (i.e. transpiration, stomatal conductance, sub-stomatal CO2 concentration). The most pronounced effects were observed for Ti 50 mg L-1 cultivation where photosynthesis efficiency, transpiration and stomatal conductance were increased by 14.69%, 4.58% and 8.92%, respectively. They were further confirmed by high maximum ribulose 1,5-bisphosphate carboxylation rate (27.40% increase), maximum electron transport rate (21.51% increase) and the lowest CO2 compensation point (45.19% decrease). Furthermore, concentrations of Cu, Mn, Zn, Fe, Mg, Ca, K and P were examined with the most pronounced changes observed for elements directly involved in photosynthesis (Cu, Zn, Mn, and Fe). The Cu concentrations in roots, stems and leaves for Ti 50 mg L-1 cultivation were below the control by 33.15%, 38.28% and 10.76%, respectively. The Zn content in analogous treatment and organs decreased by 30.24%, 26.69% and 13.35%. The Mn and Fe levels in leaves were increased by 72.22% and 50.32%, respectively. Our results indicated that plant defence mechanisms which restrain the water uptake have been overcome in pea by photocatalytic activity of nanoparticulate TiO2 which stimulated photosynthesis. On the contrary to the substantial stomatal conductance, the transpiration has been reduced because exceptional part of water flow was already consumed in chloroplasts and could not have been freed to the atmosphere.

Data on protein post-translational modifications (PTMs) increased exponentially in the last years due to the refinement of mass spectrometry techniques and the development of databases to store and share datasets. Nevertheless, these data per se do not create comprehensive biochemical knowledge. Complementary studies on protein biochemistry are necessary to fully understand the function of these PTMs at the molecular level and beyond, for example, designing rational metabolic engineering strategies to improve crops. Phosphoenolpyruvate carboxykinases (PEPCKs) are critical enzymes for plant metabolism with diverse roles in plant development and growth. Multiple lines of evidence showed the complex regulation of PEPCKs, including PTMs. Herein, we present PEPCKs as an example of the integration of combined mechanisms modulating enzyme activity and metabolic pathways. PEPCK studies strongly advanced after the production of the recombinant enzyme and the establishment of standardized biochemical assays. Finally, we discuss emerging open questions for future research and the challenges in integrating all available data into functional biochemical models.

The aim of this study was to examine the effect of the modified light spectrum of glass containing red luminophore on the performance of the photosynthetic apparatus of two types of lettuce cultivated in soil in a greenhouse. Butterhead and iceberg lettuce were cultivated in two types of greenhouses: (1) covered with transparent glass (control) and (2) covered with glass containing red luminophore (red). After 4 weeks of culture, structural and functional changes in the photosynthetic apparatus were examined. The presented study indicated that the red luminophore used changed the sunlight spectrum, providing an adequate blue:red light ratio, while decreasing the red:far-red radiation ratio. In such light conditions, changes in the efficiency parameters of the photosynthetic apparatus, modifications in the chloroplast ultrastructure, and altered proportions of structural proteins forming the photosynthetic apparatus were observed. These changes led to a decrease of CO2 carboxylation efficiency in both examined lettuce types.

Due to the photocatalytic property of titanium dioxide (TiO2), its application may be dependent on the growing light environment. In this study, radish plants were cultivated under four light intensities (75, 150, 300, and 600 μmol m-2 s-1 photosynthetic photon flux density, PPFD), and were weekly sprayed (three times in total) with TiO2 nanoparticles at different concentrations (0, 50, and 100 μmol L-1). Based on the obtained results, plants used two contrasting strategies depending on the growing PPFD. In the first strategy, as a result of exposure to high PPFD, plants limited their leaf area and send the biomass towards the underground parts to limit light-absorbing surface area, which was confirmed by thicker leaves (lower specific leaf area). TiO2 further improved the allocation of biomass to the underground parts when plants were exposed to higher PPFDs. In the second strategy, plants dissipated the absorbed light energy into the heat (NPQ) to protect the photosynthetic apparatus from high energy input due to carbohydrate and carotenoid accumulation as a result of exposure to higher PPFDs or TiO2 concentrations. TiO2 nanoparticle application up-regulated photosynthetic functionality under low, while down-regulated it under high PPFD. The best light use efficiency was noted at 300 m-2 s-1 PPFD, while TiO2 nanoparticle spray stimulated light use efficiency at 75 m-2 s-1 PPFD. In conclusion, TiO2 nanoparticle spray promotes plant growth and productivity, and this response is magnified as cultivation light intensity becomes limited.

The plant cuticle is an important plant-atmosphere boundary, the synthesis and maintenance of which represents a significant metabolic cost. Only limited information regarding cuticle dynamics is available. We determined the composition and dynamics of Clusia rosea cuticular waxes and matrix using 13 CO2 labelling, compound-specific and bulk isotope ratio mass spectrometry. Collodion was used for wax collection; gas exchange techniques to test for any collodion effects on living leaves. Cutin matrix (MX) area density did not vary between young and mature leaves and between leaf sides. Only young leaves incorporated new carbon into their MX. Collodion-based sampling discriminated between epicuticular (EW) and intracuticular wax (IW) effectively. Epicuticular differed in composition from IW. The newly synthetised wax was deposited in IW first and later in EW. Both young and mature leaves synthetised IW and EW. The faster dynamics in young leaves were due to lower wax coverage, not a faster synthesis rate. Longer-chain alkanes were deposited preferentially on the abaxial, stomatous leaf side, producing differences between leaf sides in wax composition. We introduce a new, sensitive isotope labelling method and demonstrate that cuticular wax is renewed during leaf ontogeny of C. rosea. We discuss the ecophysiological significance of the new insights.