肽核酸(PNA)是合成分子,就像DNA/RNA,但是有不同的积木。它们已被研究为潜在的抗生素,因为它们可以特异性地靶向和结合mRNA并破坏目标基因的功能。本系统综述旨在对PNA作为抗菌剂的现状进行深入分析,定义有效PNA结构的特征,并解决PNA进展中的差距,成为临床合格的药物。按照PRISMA模型,我们搜索了四个电子数据库WebofScience,PubMed,SciFinder和Scopus,在1994年至2023年之间发现了627篇发表的文章。经过筛选和严格的选择过程,使用明确的纳入和排除标准,选择了65篇科学文章,包含656个最小抑制浓度(MIC)数据。针对20种细菌物种评估了PNA的抗菌活性。研究最多的革兰氏阴性和革兰氏阳性是大肠杆菌(n=266)和金黄色葡萄球菌(n=53),分别。此外,我们揭示了PNA设计的影响,如结构长度,绑定位置,和载体剂对其抗菌活性的影响。最后,我们检查了抗菌测试模型的效果,以评估PNA的抑制作用,强调差距和前景。本系统综述全面评估了PNA作为抗菌剂的潜力,并为寻求新的治疗策略来对抗抗生素抗性细菌的研究人员和临床医生提供了有价值的见解。
Peptide nucleic acids (PNAs) are synthetic molecules that are like DNA/RNA, but with different building blocks. PNAs target and bind to mRNAs and disrupt the function of a targeted gene, hence they have been studied as potential antibacterials. The aim of this systematic
review was to provide an in-depth analysis of the current status of PNAs as antibacterial agents, define the characteristics of the effective PNA constructs, and address the gap in advancing PNAs to become clinically competent agents. Following the PRISMA model, four electronic databases were searched: Web of Science, PubMed, SciFinder and Scopus. A total of 627 articles published between 1994 and 2023 were found. After screening and a rigorous selection process using explicit inclusion and exclusion criteria, 65 scientific articles were selected, containing 656 minimum inhibitory concentration (MIC) data. The antibacterial activity of PNAs was assessed against 20 bacterial species. The most studied Gram-negative and Gram-positive bacteria were Escherichia coli (n=266) and Staphylococcus aureus (n=53), respectively. In addition, the effect of PNA design, including construct length, binding location, and carrier agents, on antibacterial activity was shown. Finally, antibacterial test models to assess the inhibitory effects of PNAs were examined, emphasising gaps and prospects. This systematic
review provides a comprehensive assessment of the potential of PNAs as antibacterial agents and offers valuable insights for researchers and clinicians seeking novel therapeutic strategies in the context of increasing rates of antibiotic-resistant bacteria.