植物交配系统塑造了遗传多样性的模式,并影响了种群的长期成功。因此,它们与旨在最大化遗传多样性的种子收集设计相关(例如,种质保护,生态恢复)。然而,对于大多数物种来说,关于交配系统和遗传多样性的变化是如何分布的,经验上知之甚少。我们调查了两个功能相似的遗传多样性和交配系统之间的关系,合生的Hakea(Proteaceae)物种,并评估了在种子中捕获遗传多样性的程度。我们通过DArTseq对数百株幼苗和母株进行了基因分型,并开发了两种从SNP数据推断交配系统的方法的新颖实现。显示了H.sericea和H.tereifolia之间遗传多样性模式的鲜明对比,与他们的交配系统的对比一致。虽然这两个物种都有混合的交配系统,H.sericea被发现习惯性地自交,而H.tereifolia更均匀地使用自交和异交。在这两个物种中,种子收集计划通过增加母系和采样地点的数量来最大化遗传多样性,但是自交物种需要两倍的站点才能在区域范围内捕获同等水平的遗传变异。
Plant mating systems shape patterns of genetic diversity and impact the long-term success of populations. As such, they are relevant to the design of seed collections aiming to maximise genetic diversity (e.g., germplasm conservation, ecological restoration). However, for most species, little is known empirically about how variation in mating systems and genetic diversity is distributed. We investigated the relationship between genetic diversity and mating systems in two functionally similar, co-occurring species of Hakea (Proteaceae), and evaluated the extent to which genetic diversity was captured in seeds. We genotyped hundreds of seedlings and mother plants via DArTseq, and developed novel implementations of two approaches to inferring the mating system from SNP data. A striking contrast in patterns of genetic diversity between H. sericea and H. teretifolia was revealed, consistent with a contrast in their mating systems. While both species had mixed mating systems, H. sericea was found to be habitually selfing, while H. teretifolia more evenly employed both selfing and
outcrossing. In both species, seed collection schemes maximised genetic diversity by increasing the number of maternal lines and sites sampled, but twice as many sites were needed for the selfing species to capture equivalent levels of genetic variation at a regional scale.