视网膜由排列在八个细胞层和两个源自神经外胚层细胞的膜中的各种细胞类型组成。在这项研究中,使用光学和电子显微镜以及免疫组织化学技术研究了细胞成分和兔视网膜各层的分化和分布时间。使用了32只兔子胚胎和12只兔子。兔视网膜在妊娠的第10天以视杯的形式开始产前发育。神经和神经胶质发生的过程分为几个阶段：在第一阶段，神经节细胞在第15天分化。第二阶段包括穆勒的分化，无碱,和第23天的视锥细胞.两极的分化，水平,视杆细胞和光感受器内部部分的形成考虑到妊娠第27天和第30天发生的晚期。出生后的第一周，光感受器的外段发育。S100蛋白由Muller细胞及其从外部到内部的穿过视网膜的过程表达。钙视网膜素在无长突和置换的无长突细胞内被强烈标记。神经节细胞对局限于其细胞质和树突的钙视网膜素表现出中等的免疫反应性。总之,兔视网膜的神经和神经胶质生成的所有阶段都发生在胚胎期。然后，视网膜在出生后通过形成光感受器外节继续发育，视网膜的所有层都建立起来。研究重点：本研究的目的是研究出生前和出生后兔视网膜的形态发生。可以以视杯的形式观察到视网膜的原基。神经节细胞是第一个分化的细胞，而感光细胞是最后一个。S100蛋白由Muller细胞及其过程表达。Calretinin在无长突和置换的无长突细胞中被强烈标记，并在神经节细胞的细胞质和树突中中等表达。
The retina consists of various cell types arranged in eight cell layers and two membranes that originate from the neuroectodermal cells. In this
study, the timing of differentiation and distribution of the cellular components and the layers of the rabbit retina are investigated using light and electron microscopy and immunohistochemical techniques. There were 32 rabbit embryos and 12 rabbits used. The rabbit retina begins its prenatal development on the 10th day of gestation in the form of optic cup. The process of neuro- and gliogenesis occurs in several stages: In the first stage, the ganglionic cells are differentiated at the 15th day. The second stage includes the differentiation of Muller, amacrine, and cone cells on the 23rd day. The differentiation of bipolar, horizontal, and rod cells and formation of the inner segments of the photoreceptors consider the late stage that occurs by the 27th and 30th day of gestation. On the first week of age postnatally, the outer segments of the photoreceptors are developed. S100 protein is expressed by the Muller cells and its processes that traverse the retina from the outer to the inner limiting membranes. Calretinin is intensely labeled within the amacrine and displaced amacrine cells. Ganglionic cells exhibited moderate immunoreactivity for calretinin confined to their cytoplasm and dendrites. In conclusion, all stages of neuro- and gliogenesis of the rabbit retina occur during the embryonic period. Then, the retina continues its development postnatally by formation of the photoreceptor outer segments and all layers of the retina become established. RESEARCH HIGHLIGHTS: The aim of this
study is to investigate the morphogenesis of the rabbit retina during pre- and postnatal life. The primordia of the retina could be observed in the form of the optic cup. The ganglionic cells are the first cells to differentiate, while the photoreceptor cells are the last. S100 protein is expressed by the Muller cells and its processes. Calretinin is intensely labeled in the amacrine and displaced amacrine cells and moderately expressed in the cytoplasm and dendrites of ganglionic cells.
