背景:用于评估萎缩性胃炎(AG)的血清生物标志物分析,胃癌前病变,对胃癌风险增加的患者的识别越来越感兴趣。目的是使用另一种方法分析血清胃蛋白酶原检测的诊断性能,化学发光酶免疫分析(CLEIA),以及其他新的潜在生物标志物。
方法:在我们之前的前瞻性研究中,被认为胃癌风险增加并接受上消化道内窥镜检查的患者的血清,多中心研究检测了胃蛋白酶原I(PGI)和II(PGII),白细胞介素-6(IL-6),人附睾蛋白4(HE-4),脂联素,铁蛋白和KrebsvondenLungen(KL-6)使用CLEIA。以组织学为参考,计算AG检测的诊断性能。
结果:总计,356名患者(162名男性(46%);平均年龄58.6(±14.2)岁),包括152与AG,包括在内。对于中度至重度语料库AG的检测,胃蛋白酶原I/II比率的敏感性和特异性分别为75.0%(95CI57.8-87.9)和92.6%(88.2-95.8),分别。对于中度至重度胃窦AG的检测,IL-6的敏感性为72.2%(95CI46.5-90.3)。胃蛋白酶原I/II比率或HE-4的组合显示在任何位置检测中度至重度AG的灵敏度为85.2%(95CI72.9-93.4)。
结论:这项研究表明,通过CLEIA进行的PG检测代表了检测语料库AG的准确方法。此外,IL-6和HE-4可能对胃窦AG的检测感兴趣。
结论:胃蛋白酶原化学发光酶免疫分析法检测萎缩性胃炎是准确的。IL-6和HE-4可能对胃窦萎缩性胃炎的检测感兴趣。
BACKGROUND: Analysis of serum biomarkers for the assessment of atrophic gastritis (AG), a gastric precancerous lesion, is of growing interest for identification of patients at increased risk of gastric cancer. The aim was to analyze the diagnostic performance of serum pepsinogen testing using another method, chemiluminescent enzyme immunoassay (CLEIA), as well as of other new potential biomarkers.
METHODS: The sera of patients considered at increased risk of gastric cancer and undergoing upper endoscopy collected in our previous prospective, multicenter
study were tested for pepsinogen I (PGI) and II (PGII), interleukin-6 (IL-6), human epididymal protein 4 (HE-4), adiponectin, ferritin and Krebs von den Lungen (KL-6) using the CLEIA. The diagnostic performance for the detection of AG was calculated by taking histology as the reference.
RESULTS: In total, 356 patients (162 men (46%); mean age 58.6 (±14.2) years), including 152 with AG, were included. For the detection of moderate to severe corpus AG, sensitivity and specificity of the pepsinogen I/II ratio were of 75.0% (95%CI 57.8-87.9) and 92.6% (88.2-95.8), respectively. For the detection of moderate to severe antrum AG, sensitivity of IL-6 was of 72.2% (95%CI 46.5-90.3). Combination of pepsinogen I/II ratio or HE-4 showed a sensitivity of 85.2% (95%CI 72.9-93.4) for the detection of moderate to severe AG at any location.
CONCLUSIONS: This
study shows that PG testing by CLEIA represents an accurate assay for the detection of corpus AG. Additionally, IL-6 and HE-4 may be of interest for the detection of antrum AG.
CONCLUSIONS: Pepsinogens testing by chemiluminescent enzyme immunoassay is accurate for the detection of corpus atrophic gastritis. IL-6 and HE-4 maybe of interest for the detection of antrum atrophic gastritis.