这项研究提出了一种创新的方法,通过引入一种新型的ZnO-蜂胶纳米复合材料(ZnO-PNC)来对抗细菌中抗生素耐药性不断升级的威胁。抗生素的过度使用,特别是在像COVID-19大流行这样的事件中,增强了细菌耐药性,需要创新的解决方案。该研究采用具有成本效益和可控的生物合成方法来生产ZnO纳米颗粒(ZnO-NP),蜂胶提取物对减少和稳定Zn2离子至关重要。然后通过掺入ZnO-NP来创建可生物降解的纳米蜂胶基质,形成ZnO-PNC。通过FT-IR和Zeta电位分析证实了结构稳定性,虽然通过TEM验证了纳米级特性,SEM,和XRD分析。各种物质的抗菌功效,包括蜂胶,纳米蜂胶,乙醇蜂胶提取物,ZnO-NP,和ZnO-PNC,针对革兰氏阴性和革兰氏阳性细菌进行评估,与28种抗生素进行比较。在测试的细菌中,铜绿假单胞菌PAO1ATCC15692对生物合成的纳米复合材料ZnO-PNC比对ZnO-NP(38毫米)和纳米胶(32毫米)更敏感(40毫米),而大肠杆菌对纳米蜂胶(0毫米)的抗性高于对ZnO-NP(31毫米)的抗性,和ZnO-PNC(34毫米)。该研究揭示了当蜂胶与绿色合成的ZnO-NPs以ZnO-PNCs的形式结合时的协同效应,显著提高了它们对所有测试细菌的效率,包括大肠杆菌等抗生素耐药菌株。纳米复合材料优于其他材料和抗生素,表现出显著的抗菌效果。SEM成像证实了ZnO-NP和ZnO-PNC对细菌细胞膜的破坏。该研究强调了整合到可生物降解材料中的ZnO-NPs的潜在应用,并强调了氧化锌-蜂胶纳米复合材料在对抗抗菌耐药性方面的重要性。总的来说,这项研究为对抗多重耐药细菌提供了一个全面的解决方案,为感染控制的新方法开辟了道路。
This study proposes an innovative approach to combat the escalating threat of antibiotic resistance in bacteria by introducing a novel ZnO-propolis
nanocomposite (ZnO-P NCs). The overuse of antibiotics, particularly during events like the COVID-19 pandemic, has intensified bacterial resistance, necessitating innovative solutions. The study employs a cost-effective and controllable biosynthesis method to produce ZnO nanoparticles (ZnO-NPs), with propolis extract crucially contributing to the reduction and stabilization of Zn2+ ions. A biodegradable nano-propolis matrix is then created by incorporating ZnO-NPs, forming the ZnO-P NCs. Structural stability is confirmed through FT-IR and Zeta potential analysis, while nanoscale properties are validated via TEM, SEM, and XRD analyses. The antimicrobial efficacy of various substances, including propolis, nano propolis, ethanolic propolis extract, ZnO-NPs, and ZnO-P NCs, is assessed against Gram-negative and Gram-positive bacteria, alongside a comparison with 28 antibiotics. Among the bacteria tested, Pseudomonas aeruginosa PAO1 ATCC15692 was more sensitive (40 mm) to the biosynthesized
nanocomposite ZnO-P NCs than to ZnO-NPs (38 mm) and nanopropolis (32 mm), while Escherichia coli was resistant to nanopropolis (0 mm) than to ZnO-NPs (31 mm), and ZnO-P NCs (34 mm). The study reveals a synergy effect when combining propolis with green-synthesized ZnO-NPs in the form of ZnO-P NCs, significantly improving their efficiency against all tested bacteria, including antibiotic-resistant strains like E. coli. The
nanocomposite outperforms other materials and antibiotics, demonstrating remarkable antibacterial effectiveness. SEM imaging confirms the disruption of bacterial cell membranes by ZnO-NPs and ZnO-P NCs. The study emphasizes the potential applications of ZnO-NPs integrated into biodegradable materials and underscores the significance of the zinc oxide-propolis
nanocomposite in countering antimicrobial resistance. Overall, this research offers a comprehensive solution to combat multidrug-resistant bacteria, opening avenues for novel approaches in infection control.