微创手术,如支气管内超声引导下经支气管针吸活检术(EBUS-TBNA),不仅要获得高质量和高质量的材料进行形态学评估,但也有足够的样本分析分子标记,以指导患者进行适当的靶向治疗。在这种情况下,世界各地的细胞病理学家应该熟悉对检测细胞学样本的最低要求。本手稿是对题为“分子分析的细胞学准备:EBUSTBNA的分析前问题”的研讨会内容的全面描述。在利物浦举行的第40届欧洲细胞病理学大会上,英国。本综述强调了用于分子分析的不同类型的细胞学基质的优点和局限性,如档案涂片,液基制剂,档案cytospin制剂和FTA(弗林德斯技术协会)卡,以及他们的技术要求/特点。这些各种类型的细胞学标本可以成功地用于广泛的分子研究,但提取的核酸的质量和数量直接依赖于这些样品的充分的分析前评估。在此设置中,细胞病理学家不仅必须熟悉不同类型的标本和相关的技术程序,而且还要正确处理微创手术提供的材料,确保有足够的材料用于通过个性化护理对患者进行精确诊断和正确管理。
Minimally invasive procedures such as endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) must yield not only good quality and quantity of material for morphological assessment, but also an adequate sample for analysis of molecular markers to guide patients to appropriate targeted therapies. In this context, cytopathologists worldwide should be familiar with minimum requirements for refereeing cytological samples for testing. The present manuscript is a
review with comprehensive description of the content of the workshop entitled Cytological preparations for molecular analysis: pre-analytical issues for EBUS TBNA, presented at the 40th European Congress of Cytopathology in Liverpool, UK. The present
review emphasises the advantages and limitations of different types of cytology substrates used for molecular analysis such as archival smears, liquid-based preparations, archival cytospin preparations and FTA (Flinders Technology Associates) cards, as well as their technical requirements/features. These various types of cytological specimens can be successfully used for an extensive array of molecular studies, but the quality and quantity of extracted nucleic acids rely directly on adequate pre-analytical assessment of those samples. In this setting, cytopathologists must not only be familiar with the different types of specimens and associated technical procedures, but also correctly handle the material provided by minimally invasive procedures, ensuring that there is sufficient amount of material for a precise diagnosis and correct management of the patient through personalised care.